目的 研究脊痛宁片有效成分与模拟生物膜的相互作用，预测药物在体内的吸收情况。方法 将脂质体作为模拟生物膜，采用脂质体平衡透析与液质联用技术筛选脊痛宁片中与脂质体有相互作用的成分，并考察脂质体质量浓度和缓冲液pH值对脊痛宁片与脂质体互相作用的影响。结果 脊痛宁片中共有11个成分与脂质体作用明显，包括4个黄酮类成分，2个萜类成分，4个香豆素类成分，1个苯丙酸类成分。脂质体的质量浓度和缓冲液pH值都对脊痛宁片与脂质体互相作用有一定的影响。结论 脂质体平衡透析与液质联用可预测脊痛宁片潜在的活性成分在体内的吸收情况，为其药效物质基础的进一步研究提供依据。

Objective To explore the interaction between the effective components of Jitongning Tablet (JT) and liposome biomembrance, in order to predict in vivo absorption of the effective components. Methods Liposome as the model of biomembrance, active components were screened by liposome equilibrium dialysis and LC-MS; And the effect of experiment conditions on the interaction of the liposome concentration, and the pH value of buffer were also investigated. Results There were 11 compounds in JT with obvious interaction with liposome, including 4 flavonoids, 2 triterpenoid, 4 coumarins, and 1 phenylpropionic acid. The concentration of liposome and pH value of buffer remarkably affected the interaction between JT and liposome. Conclusion The established method contributes to predicting the multiple components absorption in body, indicating the potentially active compounds and providing reference for further research of pharmacodynamic substance basis.

国家科技重大专项课题——面向国际的创新中药大平台建设（2013ZX09402202）