目的 采用超高效液相色谱-四极杆飞行时间质谱（UPLC-Q-TOF/MS）联用技术对核桃楸皮进行分析，确定其主要化学组分。方法 采用Waters Acquity UPLC BEH C₁₈色谱柱（100 mm×2.1 mm，1.7 μm），以0.1%甲酸水（A）-0.1%甲酸乙腈（B）为流动相梯度洗脱；质谱采用电喷雾（ESI）离子源，在正负离子模式下采集数据，通过Peakview 2.0/masterview1.0及Markerview1.2.1软件进行目标及非目标筛查化合物，依据精确质量数和同位素峰度比确定化合物分子式，通过对照品及数据库的二级谱图比对、裂解规律分析，结合已有文献报道，确定化合物结构式。结果 鉴定或推断了核桃楸皮中44种主要化学成分，其中包括13个萘醌类化合物，3个二芳基庚烷类化合物，15个黄酮类化合物及13个其他类化合物。结论 该方法快速、准确，为核桃楸皮的化学成分鉴定提供一种新的策略，主要化学成分的确定为质量评价指标选择及药理活性研究奠定了基础。

Objective To identify the major chemical constituents in Cortex Juglandis Mandshuricae by ultra performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-Q-TOF/MS). Methods The separation was performed on Waters Acquity UPLC BEH C₁₈ column (100 mm×2.1 mm, 1.7 μm), with a mobile phase using water with 0.1% formic acid (A) and acetonitrile with 0.1% formic acid (B) for gradient elution; Q-TOF/MS and electrospray ion (ESI) source was applied for the analysis under the positive and negative ion modes; Target search and non-target search were performed by Peakview 2.0/masterview1.0 or Markerview 1.2.1 software. Then the formulas of compounds were determined by accurate mass and isotopic abundance ratio from target screening function of software. Their structures were determined by analysis of MS/MS fragment or comparison with standard substances and references. Results Forty-four major chemical compounds including 13 naphthalene quinones, 3 diarylheptanoids, 15 flavonoids, and 13 other compounds were identified or inferred in Cortex Juglandis Mandshuricae. Conclusion UPLC-Q-TOF/MS method which develops a new strategy can identify the main chemical constituents from Cortex Juglandis Mandshuricae rapidly and accurately, main chemical constituents can be used for quality evaluation and pharmacological research.

国家自然科学基金项目（81503348）；国家“重大新药创制”科技重大专项（2010ZX09102-138）；哈尔滨市青年科技创新人才基金项目（2012RFOYSO42）