目的 采用¹H-NMR代谢组学方法，探讨黄芩素对D-半乳糖致衰老模型大鼠的干预作用。方法 采用sc D-半乳糖（150 mg/kg）致大鼠衰老模型，考察黄芩素（50、100、200 mg/kg）对衰老大鼠自主活动能力的影响。造模10周后采集大鼠血清进行¹H-NMR检测，结合多元统计分析探讨黄芩素的抗衰老作用。结果 黄芩素能显著提高衰老大鼠的自主活动能力。与对照组相比，模型组大鼠血清中丙氨酸、醋酸、丙酮酸、酪氨酸、N-乙酰糖蛋白水平升高，而谷氨酰胺、二甲基甘氨酸、甘氨酸、苏氨酸、肌酐、组氨酸水平降低，给予黄芩素后能不同程度回调这11种潜在的生物标志物。结论 黄芩素可能通过调控氨基酸代谢、核苷酸代谢和糖代谢等途径延缓衰老。

Objective To explore the intervention of baicalein on D-galactose-induced aging rats by ¹H-NMR metabonomics methods. Methods The aging model was induced by sc injection of D-galactose (150 mg/kg) in rats, and the effects of baicalein (50, 100, and 200 mg/kg) on autonomic activities of aging rats were investigated. After 10 weeks, the serum of rats was collected for ¹H-NMR detection, and the anti-aging effects of baicalein were explored using multivariate statistical analysis. Results Baicalein significantly improved the locomotor activities of aging rats. Compared with control group, the levels of alanine, acetic acid, pyruvic acid, tyrosine, and N-acetyl glycoproteins in rat serum of model group increased, while the levels of glutamine, dimethyl glycine, glycine, threonine, creatinine, and histidine decreased. These 11 potential biomarkers could be reversely regulated after treatment with baicalein. Conclusion Baicalein exerts anti-aging effects likely through regulation of amino acid metabolism, nucleotide metabolism, and glucose metabolism.

国家自然科学基金资助项目（81603319）；山西大学引进人才事业发展经费（226545008，226545003）；2015年度山西省高等学校科技创新项目（2015118）