目的 通过气相色谱-质谱联用（GC-MS）技术分析呼吸道合胞病毒（RSV）感染小鼠脾脏组织中内源性代谢物的变化，探讨金欣口服液治疗RSV肺炎可能的作用机制。方法 RSV滴鼻感染BALB/c小鼠建立RSV肺炎模型，给予金欣口服液（27.6 g/kg）干预治疗，连续治疗7 d后处死小鼠，取脾脏组织，经甲醇提取后，先后予甲氧胺吡啶和BSTFA以肟化和衍生化，然后运用GC-MS检测脾脏中所含有的代谢物及其相对量的变化。结果 RSV肺炎小鼠的脾脏中共鉴定出35种代谢产物，其中L-脯氨酸、L-谷氨酸、缬氨酸、尿素、次黄嘌呤、葡萄糖为具有显著性差异的代谢物（P<0.05），与之相关的代谢通路为D-谷氨酰胺和谷氨酸代谢，缬氨酸、亮氨酸和异亮氨酸代谢，丙氨酸、天冬氨酸和谷氨酸代谢，精氨酸和脯氨酸代谢。结论 金欣口服液可以调节RSV肺炎小鼠脾脏的免疫功能，可能与氨基酸代谢有关。

Objective To detect the relative abundance changes of metabolites in spleen to explore the antiviral effect of Jinxin Oral Liquid (JOL) against RSV pneumonia by GC-MS technology. Methods BALB/c mice were challenged intranasally to establish the RSV pneumonia models and ig administered with JOL (27.6 g·kg^-1·d^-1). After treated for 7 d, mice spleens were collected respectively. Metabolites were extracted by methanol, and then oximated, derevatized, and detected by GC-MS. Results 35 metabolites in spleen were identified, among which L-proline, L-glutamic acid, valine, urea, hypoxanthine, glucose were statistically significant (P<0.05). Conclusion Anti-RSV infection of JOL may be associated closely with the regulation of immune function of mice spleen, and amino acid metabolism is involved.

国家自然科学基金资助项目（81373688，81303295）；江苏省研究生培养创新工程（SJZZ15_0120）