目的 研究通关藤Marsdenia tenacissima种子检验方法，为制定通关藤种子检验规程及质量分级提供依据。方法 以当年采收的不同产地通关藤种子为材料，参照国际植物种子检验规程和中国农作物种子检验规程，对其质量进行检验，探索通关藤种子质量检验的方法。结果 通关藤种子净度分析送检样本至少900 g，实验样品至少90 g；种子千粒质量测定选用五百粒法；含水量测定选用高恒温（133±2）℃烘干法，烘干时间为6 h；发芽前自来水浸种24 h，于砂中在30℃光照条件下做发芽实验，发芽计数时间为1~8 d；生活力测定采用0.1% TTC溶液染色，于恒温35℃黑暗条件下染色3 h。结论 初步建立了包括扦样、净度分析、真实性鉴定、千粒质量、发芽条件、含水量及生活力7项指标在内的通关藤种子质量检验方法。

Objective To optimize the testing methods for seed quality of Marsdenia tenacissima,and provide basis for establishing seed quality standard of M.tenacissima.Methods The seed quality of M.tenacissima from different producing areas was measured based on the International Seed Testing Protocol made up by ISTA and Rules for Agricultural Seed Testing issued by China.Results The samples weight of M.tenacissima were at least 900 g for purity analysis and were at least 90 g for testing.The 1 000-seed weight was determined by 500-seed method,and the water content was carried out by higher temperature (133±2)℃ for 6 h.After soaking in water for 24 h,M.tenacissima seeds were cultured in wet sand at 30℃ for 1-8 d under illumination for germination testing.Seed viability was tested by TTC method in 35℃ for 3 h.Conclusion The seed testing methods for quality items of M.tenacissima have been initially established.