目的 以云南省不同地区云南重楼及多芽品系为材料，采用UPLC-ELSD法同时测定7种甾体皂苷量并建立高黎贡山地区样品UPLC指纹图谱，评价其质量。方法 采用UPLC-ELSD法，ACQUITY UPLC BEH C₁₈（50 mm×2.1 mm，1.7 μm）色谱柱，体积流量0.2 mL/min，漂移管温度55℃，氮气压力275.8 kPa，增益500，以乙腈-水为流动相梯度洗脱。结果 样品在23 min内能完全被分离，方法学考察均符合要求；23份样品中19份符合《中国药典》2015年版要求，《中国药典》项下4个皂苷平均质量分数为1.42%，其中高黎贡山地区的样品平均质量分数高达1.660%；10批次高黎贡山地区云南重楼（多芽品系）样品间相似度小于0.9，标定了8个共有特征峰，并对4个特征峰进行了归属；样品中7个皂苷的种类和量经PCA分析和系统聚类分析均不能明显分类。结论 该方法简便、快速、灵敏度高，可用于云南重楼的质量快速评价；云南重楼及其多芽品系在化学成分种类和量上并无明显区别；高黎贡山地区的云南重楼（多芽品系）是一个值得推广栽培和深入研究的品种。

Objective To establish a quantitative analysis method of seven steroidal saponins in Paridis Rhizoma and its polygerm varieties from different regions in Yunnan province,to simultaneously determine the contents of seven steroidal saponins,to establish the fingerprint for the polygerm varieties in Gaoligong Mountain by UPLC-ELSD,and to evaluate the qualities.Methods It was detected with an Acquity UPLC^® BEH C₁₈(2.1 mm×50 mm,1.7 μm) column,and gradually diluted with acetonitrile-water at a flow rate of 0.2 mL/min,drift tube temperature of 55℃,nitrogen pressure 275.8 kPa,and gain 500 by UPLC-ELSD.Results The 23 samples could be separated and analyzed of seven steroids within 23 min.All the indexes of the methodological investigation met the requirements.The results showed that the contents of saponins from 19 samples met the requirement in Chinese Pharmacopoeia 2015.The average content of four saponins was 1.42% and among them the content from the sample in Gaoligong Mounta was up to 1.660%.The similarity was lower than 0.9 in the samples of Gaoligong Mountain,and there were eight common peaks in the fingerprints,which were identified four characteristic peaks.The type and content of seven saponins in the samples could not be clearly classified by PCA analysis and system cluster analysis.Conclusion The method is convenient,accurate,and suitable for the quantitative analysis of Paridis Rhizoma and polygerm varieties.There is no obvious difference between Paridis Rhizoma and polygerm varieties in chemical components and contents.Paridis Rhizoma (polygerm varieties) in Gaoligong Mountain is a variety worth popularization and in-depth researches.

四川省科技支撑计划（15ZC0473）；四川省重点技术创新项目计划（2014XM041）；阿坝州应用技术研究与开发资金（2015）