目的 研究甘草Glycyrrhizae Radix et Rhizoma中的抗炎活性成分。方法 采用大孔树脂、ODS、半制备液相等色谱手段进行分离纯化，并通过LC-MS、¹H-NMR、¹³C-NMR等波谱技术进行结构鉴定。用细菌脂多糖（LPS）诱导小鼠巨噬细胞RAW264.7炎症模型对分离到的化合物进行抗炎活性筛选。 结果 从甘草中分离得到10个化合物，分别鉴定为甘草苷（1）、芹糖甘草苷（2）、异甘草苷（3）、芹糖异甘草苷（4）、sophoraisoflavone A（5）、粗毛甘草素F（6）、光甘草酮（7）、光甘草定（8）、甘草黄酮醇（9）和粗毛甘草素D（10）。结论 化合物1、3、5、6、8和9对LPS诱导的RAW264.7细胞NO分泌有一定的抑制作用。其中化合物5、6和9抑制LPS诱导的RAW 264.7细胞NO分泌为首次报道。

Objective To study the anti-inflammatory constituents in Glycyrrhizae Radix et Rhizoma.Methods The compounds were isolated and purified by means of macroporous resin,ODS column chromatography,and semi-preparative HPLC.And their structures were identified by LC-MS,¹H-NMR,and ¹³C-NMR.The anti-inflammatory activities of the compounds were evaluated on LPS-induced NO production in RAW 264.7 macrophages.Results Their structures were identified as liquiritin (1),liquiritin apioside (2),isoliquiritin (3),isoliquiritin apioside (4),sophoraisoflavone A (5),glyasperin F (6),glabrone (7),glabridin (8),licoflavonol (9),and glyasperin D (10).Conclusion Compounds 1,3,5,6,8,and 9 could inhibit NO production in RAW 264.7 macrophages to some extent.And the anti-inflammatory effects of compounds 5,6,and 9 are first reported in this work.

国家“重大新药创制”科技重大专项（2013ZX09402203）