目的 研究荆条Vitex negundo var. heterophylla枝条的化学成分。方法 使用硅胶柱色谱、Sephadex LH-20及制备高效液相色谱法等手段进行分离纯化，通过光谱数据结合参考文献鉴定化合物结构。结果 从荆条枝条95%乙醇提取物的正丁醇萃取部分分离得到8个黄酮苷类化合物，分别鉴定为芹菜素-6-C-β-D-吡喃葡萄糖-8-C-α-L-阿拉伯糖苷（1）、异荭草苷（2）、木犀草素-6-C-α-L-阿拉伯糖-8-C-β-D-吡喃葡萄糖苷（3）、木犀草素-6,8-2-C-α-L-阿拉伯糖苷（4）、芹菜素-6-C-α-L-阿拉伯糖-8-C-β-D-吡喃葡萄糖苷（5）、木犀草素-6-C-α-L-阿拉伯糖-8-C-β-L-阿拉伯糖苷（6）、木犀草素-6-C-β-L-阿拉伯糖-8-C-β-D-吡喃葡萄糖苷（7）、木犀草素-7-O-β-D-葡萄糖苷（8）。结论 化合物1、3~7为首次从牡荆属植物中分离得到。

Objective To study the chemical constituents in the twigs of Vitex negundo var. heterophylla.Methods A variety of silica gel column chromatography,Sephadex LH-20 gel column chromatography,and HPLC methods were used for the separation and purification of chemical composition.Their structures were identified on the basis of physicochemical property and spectral data.Results Eight compounds were obtained and identified as apigenin-6-C-β-D-glucopyranosyl 8-C-α-L-arabinopyranoside (1),isoorientin (2),luteolin-6-C-α-L-arabinopyranosyl-8-C-β-D-glucopyranoside (3),luteolin-6,8-di-C-α-L-arabinopyranoside (4),apigenin-6-α-L-arabinopyranosyl-8-C-β-D-glucopyranoside (5),luteolin-6-C-α-L-arabinopyranosyl 8-C-β-L-arabinopyranoside (6),luteolin-6-C-β-L-arabinopyranosyl-8-C-β-D-glucopyranoside (7),and luteolin-7-O-β-D-glucopyranoside (8).Conclusion Compounds 1 and 3-7 are first isolated from the plants of Vitex Linn.

锦州医科大学青年启动基金（Y2011Z023）