目的 对羊脆木Pittosporum kerrii根皮的化学成分进行研究。方法 运用硅胶、凝胶及RP-HPLC等多种色谱技术进行分离纯化，并根据理化性质和波谱数据鉴定化合物的结构。结果 从羊脆木根皮70%丙酮提取物中分离鉴定了3个异苯并呋喃内酯类化合物，分别为5-羟基-6-异戊烯基-异苯并呋喃-1（3H）-酮（1）、5-甲基-6-异戊烯基-异苯并呋喃-1（3H）-酮（2）、5-甲氧基-6-（3-O-乙酰基）-丙酰基-异苯并呋喃-1（3H）-酮（3）。其中化合物1为新化合物，命名为羊脆木素A。并对化合物1进行了细胞毒活性测试，其对NB4、SH-SY5Y、PC3、A549和MCF-7细胞的IC₅₀值分别为3.6、5.2、8.8、5.7和6.0 μmol/L。结论 化合物1~3均为首次从羊脆木中分离得到，化合物1为新化合物，且表现出细胞毒活性。

Objective To research the chemical constituents in the root barks of Pittosporum kerrii.Methods The chemical constituents in the root barks of P.kerrii were isolated by silica gel,Sephadex LH-20 column chromatographies,and RP-HPLC methods.Their structures were elucidated using various spectroscopic techniques,including extensive 1D and 2D NMR techniques.Results Three isobenzofuranlactones were isolated from the 70% acetone extract in the root barks of P.kerrii.They were 5-hydroxy-6-prenyl-isobenzofuran-1(3H)-one (1),5-methyl-6-prenyl-isobenzofuran-1(3H)-one (2),and 3-(6-methoxy-3-oxo-1,3-dihydroisobenzofuran-5-yl)-3-oxopropyl acetate (3).Conclusion Compounds 1-3 are isolated from P.kerrii for the first time.Compound 1 is a new compound and it is identified as 5-hydroxy-6-prenyl-isobenzofuran-1(3H)-one,named as kerriin A.And it displays obvious cytotoxicity against NB4,SH-SY5Y,PC3,A549,and MCF7 cells with IC₅₀ values of 1.8,2.6,3.5,4.2,and 2.3 μmol/L,respectively.

国家自然科学基金资助项目（21362044，21032085）；云南省高校科技创新团队资助项目（IRTSTYN2014-11）