目的 探讨鹿骨多肽对地塞米松诱导的骨质疏松模型大鼠骨微结构的影响。方法 以im地塞米松诱导糖皮质激素性骨质疏松（glucocorticoid-induced osteoporosis，GIOP）模型。同时ig不同剂量（250、125、62.5 mg/kg）的鹿骨多肽，连续75 d。检测血清生化指标；采用microCT方法对胫骨近端骨小梁结构进行三维分析；HE染色观察股骨远端组织病理形态。结果 鹿骨多肽可以增加GIOP模型大鼠血清Ca²⁺、骨钙素（BGP）水平，使血清P³⁺、碱性磷酸酶（ALP）和甲状旁腺激素（PTH）水平下降。鹿骨多肽虽有降低GIOP模型大鼠血清降钙素（CT）的趋势，但与模型组比较没有统计学意义。GIOP大鼠骨小梁变细，中断、游离端增多，数量减少，间隙增宽，空间结构破坏增加，而鹿骨多肽的干预能明显改善上述骨显微结构指标。结论 鹿骨多肽能够抑制地塞米松诱导的钙磷代谢失衡，降低ALP，升高BGP，抑制骨吸收和促进骨形成，并且改善GIOP大鼠病理学改变和显微结构，对骨质疏松大鼠具有保护作用。

Objective To investigate the effect of deer bone polypeptides on bone microarchitecture of dexamethasone-induced osteoporosis (GIOP) rats. Methods The rats were im given dexamethasone for establishing a rat GIOP model. The rats were ig given deer bone polypeptide with different doses for 75 d. The serum biochemical indexes were classic detection. The three-dimensional analysis of the trabecular structure of proximal tibia was conducted by microCT method; The pathological changes in the morphology of distal femur were observed by hematoxylin. Results Deer bone polypeptides could increase serum Ca²⁺ and bone-gla-protein (BGP), decrease serum P³⁺, alkaline phorphatase (ALP), and parathyroid (PTH) in rats with GIOP. Deer bone polypeptide showed a tendency to reduce the serum CT in rats with GIOP, but the decreasing tendency was not statistically significant compared with the blank control group. The trabecula was thin and ruptured, its free ends increased, its number decreased, its spaces were widened, and the destructed space structures increased in rats with GIOP, while the intervention with deer bone polypeptides could significantly improve the above indexes to evaluate the bone microstructure. Conclusion Deer bone polypeptides can inhibit the glucocorticoids-induced metabolism imbalance of calcium and phosphorus, reduce the serum ALP, increase the serum BGP, and inhibit the bone resorption and bone formation in rats with GIOP. Moreover, deer bone polypeptides can improve pathological changes in the microstructure and protect osteoporosis rats.

吉林省科技发展计划项目（20130727008YY）；长白山药用动植物活性多肽研究与开发国家地方联合工程实验室（发改高技2015-3173号）