查阅近年国内外相关文献，对血管内皮细胞连接及益气中药黄芪有效成分对血管内皮细胞连接的保护机制进行归纳总结。黄芪多糖和黄芪皂苷均可通过上调紧密连接（tight junctions，TJ）相关蛋白表达、下调细胞黏附分子（cell adhesion molecules，CAM）表达来增加内皮细胞连接的完整性，还可通过抑制细胞内皮素（ET）分泌、增加NO释放、抑制细胞凋亡和促进血管生成中内皮细胞的增殖和迁移等保护内皮细胞功能。而黄芪黄酮则主要通过抑制内皮细胞凋亡保护内皮细胞。黄芪有效成分对内皮细胞的保护作用涉及p38 MAPK/NF-κB、Rho/ROCK、PI3K/Akt、VEGF/Ang-1等信号通路多个关键性靶点，目前的研究主要集中在对TJ和CAM抑制单核细胞与内皮细胞黏附调控上，少有涉及缝隙连接（gap junctions，GJ）的保护作用研究。

To summarize the research progress in vascular endothelial cells junction as well as mechanism of effective components in Yiqi Chinese medicine Astragali Radix in vascular endothelial cells junction based on referring literature in recent year. Astragaluspolysaccharides and Astragalus saponins can protect the integrity of endothelial cells junction by increasing tight junction protein and decreasing endothelial cell adhesion molecule. Its protection takes effect by inhibiting endothelin secretion, increasing NO release, inhibiting apoptosis, and promoting endothelial cells proliferation and migration in angiogenesis in addition, while Astragalus flavonoids inhibits apoptosis to protect the junction of endothelial cells. The multiple-targeted protective effect of Astragali Radix in vascular endothelial cells involved in p38 MAPK/NF-κB, Rho/ROCK, PI3K/Akt, and VEGF/Ang-1 signaling pathways. The current study focused on tight junctions as well as adhesion molecule inhibition of monocyte adhesion to endothelial cells. But its protection of gap junctions has rarely been studied.

国家自然科学基金面上项目（8157101959）；国家自然科学基金资助项目（81073084）；重庆市自然科学基金项目（CSTC 2010BB5127）；西南大学基本科研业务费（XDJK2015C155）；西南大学博士基金（SWU114055）；教育部中央高校重点项目（XDJK2012B010）