目的 建立同时测定鸡骨香Croton crassifolius中6个萜类成分（chettaphanin I、山藿香定、crassifolin B、cyperenoic acid、crassifolin A、cyperenol）的HPLC方法。方法 采用Kromasil 100-5 C₁₈色谱柱（250 mm×4.6mm，5μm）；以乙腈（A）-0.02%三氟乙酸水（B）梯度洗脱：0~35min，35% A；35~55min，35%~60% A；55~80 min，60% A。体积流量为1.0 mL/min；检测波长为210 nm；柱温为25℃。结果 6个萜类化合物均有良好的分离度，线性关系良好（r>0.999 7），chettaphanin I、山藿香定、crassifolin B、cyperenoic acid、crassifolin A、cyperenol的加样回收率分别为100.2%、99.13%、98.48%、99.22%、101.1%、102.5%，RSD分别为0.48%、0.48%、0.96%、1.10%、1.35%、0.95%。结论 该方法简单准确，具有良好的重复性和稳定性，可为鸡骨香质量控制提供科学依据。

Objective To establish an HPLC method for simultaneous determination of chettaphanin I, teucvidin, crassifolin B, cyperenoic acid, crassifolin A, and cyperenol in Crotonis Crassifolii Radix.Methods The separation was performed on a Kromasil 100-5 C₁₈ column (250 mm × 4.6 mm, 5 μm) with acetonitrile (A)-0.02% trifluoroacetic acid water (B) as the mobile phase in a gradient elution (0—35 min, 35% A; 35—55 min, 35%—60% A; 55—80 min, 60% A) at a flow rate of 1.0 mL/min. The detective wavelength was set at 210 nm, and the column temperature was set at 25℃.Results The six terpenoids all have good separating degree and good linearity (r>0.999 7). The sample recoveries of compounds1—6 were 100.2% (RSD= 0.48%), 99.13% (RSD= 0.48%), 98.48% (RSD= 0.96%), 99.22% (RSD= 1.10%), 101.1% (RSD= 1.35%), and 102.5% (RSD= 0.95%), respectively.Conclusion The established method is rapid and accurate, and has high repeatability, which could provide the scientific evidence for the quality control of Crotonis Crassifolii Radix.

广东省优秀青年教师培养计划（Yq2013045）