目的 比较中国当归Angelica sinensis、日本当归A. acutiloba、韩国当归A. gigas、欧当归Levisticum officinale和圆当归A.archangelica药材中多糖的量，评价这5种当归类药材的质量。方法 从不同国家收集了46份当归类药材样品，采用优化的3，5-二硝基水杨酸法测定多糖量；比较不同当归药材之间多糖量的差异。结果 这5种药材中多糖的量差异很大，其平均量从高到低的顺序为圆当归（162.5mg/g）、欧当归（142.3mg/g）、日本当归（126.8mg/g）、韩国当归（81.38mg/g）、中国当归（80.75mg/g）。从日本、中国和韩国收集的日本当归中多糖量也不同，分别为131.37、184.29、94.98mg/g。结论 建立的3，5-二硝基水杨酸法可准确测定当归类药材中多糖的量；这5种药材中多糖的量不同，其功效也不同，不宜混用。

Objective Comparing the quality among five species of Angelica e Radix(Danggui) samples, i.e. Chinese Danggui (CDG, the roots of Angelica sinensis), Japanese Danggui (JDG, the roots of A. acutiloba), Korean Danggui (KDG, the roots of A. gigas), Lovage root (LR, the roots of Levisticum officinale), and Angelica archangelica root (AAR, the roots of A. archangelica) by evaluating the contents of their bioactive components.Methods The 3,5-dinitrosalicylic acid colorimetric method (DNS method) was developed to quantify the amount of polysaccharides in a total of 46 species of Angelica e Radix collected from different countries based on the optimization of sampling, comparing the differences of polysaccharide among various species of Angelica e Radix, etc.Results The contents of polysaccharides were found to be significantly different among the five species of Angelica e Radix. The order from high to low amount was AAR (162.5 mg/g, n= 9), LR (142.3 mg/g, n= 4), JDG (126.8 mg/g, n= 13), KDG (126.8 mg/g, n= 13), and CDG (80.75 mg/g, n= 12). For JDG sample, the contents of polysaccharide were significant variety among samples cultivated in Japan (131.37 mg/g, n= 3), China (184.29 mg/g, n= 4), and Korea (94.98 mg/g, n= 4).Conclusion The developed DNS method is suitable to accurately quantify the amount of polysaccharide in Angelica e Radix. The pharmaceutical efficacy is variety among the five Angelica e Radix resulting from the various contents of polysaccharide. These herbs can not be mixed or substituted in clinical use.

国家自然科学基金项目（81073044）；国家基础科学人才培养基金课题（J1310034-02）