目的 基于高效液相色谱-四级杆-飞行时间质谱联用（HPLC-DAD-Q/TOF MS）方法，通过测试大量样本获取相关数据，分析食用黄花菜中是否含有秋水仙碱。方法 将不同来源黄花菜样品分部位（根、花葶、叶、花）处理，利用HPLC-DAD-Q/TOF MS对处理样进行秋水仙碱的检测。结果 156个食用黄花菜样本中均未检出秋水仙碱，而秋水仙球样本则被检出；另外，发现黄花菜样本液相色谱图中显示与秋水仙碱对照品保留时间非常接近处有一未知峰，对该未知峰的质谱图进行分析，初步显示其为多个化合物的共流出组分，其中主要离子质荷比为m/z 455.145 5（推测分子式C₂₃H₂₂N₂O₈）。结论 食用黄花菜中不含秋水仙碱，传统认为鲜食黄花菜会导致腹泻是秋水仙碱引起的结论值得商榷。

Objective Based on HPLC-DAD-Q/TOF MS method,to analyze whether there is any colchicine in Hemerocallis citrina(HC) through large amounts of sample tests and data analyses.Methods HC from different origins were divided into different parts,and the technique of HPLC-DAD-Q/TOF MS was applied to detecting the contents of colchicine in these parts.Results Colchicine can be found in colchicum ball but cannot be found in 156 edible HC samples.From liquid chromatography of HC samples,it is also estimated that the retention time of colchicine is very close to the place where there is an unknown peak.By analyzing this peak,it can initially be recognized as the common flow part of various compounds.Among them,the major ion and mass ratio is m/z 455.145 5(speculative molecular formula of C₂₃H₂₂N₂O₈).Conclusion There may be no colchicine in HC,and the traditional view is debatable that diarrhea caused by eating fresh HC is due to colchicine.

国家科技支撑计划项目（2012BAI29B04）