目的 测定文山产三七Panax notoginseng茎、叶、花、果实、果梗和根中三七皂苷Fc的量，为三七皂苷Fc原料的制备和条件选择提供依据。方法 色谱柱为Agilent C₁₈（250 mm×4.6 mm，5 μm）；流动相为甲醇-水（65∶35）；柱温35℃；体积流量1.0 mL/min；检测波长203 nm；进样体积10 μL。采用HPLC-UV法测定三七不同部位提取物（以乙醇为溶剂，采用超声法提取）中三七皂苷Fc的量。结果 三七皂苷Fc在0.005～1.000 mg/mL线性关系良好，在三七茎、叶、花、果实、果梗和根中三七皂苷Fc的质量分数分别为0.07%、1.58%、2.13%、0.39%、0.72%和0.02%，其回收率分别为98.86%、97.43%、101.03%、99.35%、99.13%和98.68%，RSD分别为1.90%、1.87%、1.80%、1.82%、1.94%、1.88%。在此条件下，三七芦头中未检测出三七皂苷Fc。结论 三七皂苷Fc存在于文山产三七不同部位中，其中三七叶和花中三七皂苷Fc的量较高，分别为1.58%和2.13%。该研究结果可为三七叶和花的综合利用和Fc的制备应用提供参考。

Objective To determine the contents of notoginsenoside Fc in the stems,leaves,flowers,fruits,fruit stalks,and roots of Panax notoginseng in Wenshan,and provide the scientific basis for the selection of raw materials and preparation.Methods Using chromatographic column Agilent C₁₈(250 mm×4.6 mm,5 μm);mobile phase was CH₃OH-H₂O(65:35);Column temperature was 35℃;Flow rate was 1.0 mL/min;UV detection wavelength was 203 nm;injection volume was 10 μL.Using methanol as the solvent and ultrasonic extraction to extract the medicinal materials,then using the HPLC-UV method to determine the contents of notoginsenoside Fc in different parts of P.notoginseng.Results Fc had a good linear relationship when the concentration was 0.005-1.000 mg/mL.The Fc contents in the cauline,leaves,flowers,fruits,fruit stalks,and roots of P.notoginseng were 0.066%,1.58%,2.13%,0.39%,0.72%,and 0.019%.The recovery rates were 98.86%(RSD=1.90%),97.43%(RSD=1.87%),101.03%(RSD=1.80%),99.35%(RSD=1.82%),99.13%(RSD=1.94%),and 98.68%(RSD=1.88%).Under this condition,Fc was not detected in the basal part of stems of P.notoginseng.Conclusion Fc exists in different parts of P.notoginseng in Wenshan,and the contents of notoginsenoside Fc in the flowers and leaves of P.notoginseng were higher,1.58% and 2.13%,respectively.The results can provide a scientific reference for the comprehensive utilization of the leaves and flowers of P.notoginseng and the preparation and application of Fc.

中药标准提取物的工业化色谱制备平台建设（2009ZX09301-012-105B）；天然活性成分的工业化色谱制备公共服务平台（2011412004-7）；辽宁省高等学校杰出青年学者成长计划（LJQ2015108）