目的 建立白芷药材-饮片-提取物及配方颗粒的HPLC特征图谱，对其相关性进行评价。方法 采用HPLC法，色谱柱为Agilent HC-C₁₈柱（250 mm×4.6 mm，5 μm）；以乙腈-水为流动相梯度洗脱，检测波长320 nm，体积流量1.0 mL/min，柱温：25℃。以特征图谱为对照，对药材-饮片-提取物及配方颗粒进行相关性评价。结果 白芷10批药材、饮片、提取物及配方颗粒特征图谱均呈现10个共有特征峰，呈良好的相关性。结论 建立了白芷药材-饮片-提取物及配方颗粒的HPLC特征图谱，反映了白芷药材-饮片-提取物及配方颗粒多成分的整体面貌，为白芷配方颗粒质量控制提供借鉴。

Objective To establish an HPLC characteristic spectrum of raw herbs-decoction pieces-extract-dispensing granules of Angelicae Dahuricae Radix(ADR) and evaluate the correlation among them.Methods Gradient elution was performed with mobile phase of acetonitrile-water,detection wavelength of 320 nm,flow rate of 1.0 mL/min,and column temperature of 25℃,by setting the characteristic spectrum as control and evaluating the correlation of raw herbs-decoction pieces-extract-dispensing granules.Results There were 10 common characteristic peaks in all characteristic spectra of 10 batches of raw herbs,decoction pieces,extract,and dispensing granules of ADR,presenting a good correlation.Conclusion This study established an HPLC characteristic spectrum and reflected the whole picture of multi-component of raw herbs,decoction pieces,extract,and dispensing granules of ADR provides the reference for quality control of dispensing granules of ADR.

国家中医药管理局中医药科学技术研究专项（2013ZX02）