目的 观察健脾消癌方对结直肠癌裸鼠转移模型组织中血管内皮生长因子（VEGF）及血管内皮抑素（ES）表达的影响，探讨健脾消癌方拮抗结直肠癌转移的作用机制。方法 将BALB/c-nu裸鼠按随机数字表随机抽取8只作为对照组，其余裸鼠采用盲肠内注射50 μL人结肠癌HCT116细胞造模，3 d后取存活裸鼠32只，随机分为健脾消癌方低、高剂量（10、40 g/kg）组，5-氟尿嘧啶（5-FU，0.13 g/kg）组，模型组，分别ig 或ip相应药物及生理盐水4周后，采用qRT-PCR法测定裸鼠结直肠癌转移后结肠、肝、肺及盲肠肿瘤组织中VGEF、ES mRNA表达量。结果 在VEGF mRNA表达量方面，各组均较对照组升高，模型组与5-FU组最高（P<0.01）；健脾消癌方2组较模型组显著降低（P<0.05、0.01）。在ES mRNA表达量方面，各组均较对照组降低，模型组与5-FU组最低（P<0.01）；健脾消癌方2组较模型组显著升高（P<0.05、0.01）。4个部位以盲肠组织VEGF水平最低，ES水平基本相当。结论 健脾消癌方可能通过使结肠、肝、肺等组织VEGF的降低与ES的升高来达到拮抗结直肠癌转移的目的。

Objective This article is aimed at investigating the effect of Jianpi Xiaoai Recipe (JXR) on the mRNA expression of vascular endothelial growth factor (VEGF) and endostation (ES) in the tissue in nude mice with colorectal cancer metastasis. Methods Eight BALB/c-nu nude mice were randomly selected as the control group. The remaining mice, after establishing the metastatic tumor by injecting in the appendix of 50 μL human colon carcinoma HCT116 cells, then 32 survival nude mice were taken after 3 d, and divided into JXR (low- and high-dose, 10 and 40 g/kg), 5-FU (0.13 g/kg), and model groups. After the mice were ig or ip given corresponding medicine and physiological saline for 4 weeks, the mRNA expression levels of VGEF and ES in the metastatic tumor tissue of colon, liver, lung, and cecum were evaluated by qRT-PCR method. Results The mRNA expression of VEGF was higher in all groups except the control group, and the highest was in the model and 5-FU groups (P < 0.01). Compared with the model group, the mRNA expression level in two JXR groups were significantly lower (P < 0.05, 0.01). ES transcription expression was lower in each group than that in the control group, the lowest was in the model and 5-FU groups (P < 0.01). Compared with the model group, the mRNA expression levels in the two JXR groups were significantly higher (P < 0.05, 0.01). Among these four organs, the expression level of VEGF in cecum was lowest, while the mRNA expression levels of ES are nearly the same. Conclusion JXR is possible to make the mRNA expression level of VEGF decreased, but the mRNA expression level of ES increased in the metastatic tumor tissue of colon, liver, lung, and cecum, so as to resist the metastasis of colorectal cancer.

国家自然科学基金面上项目（81373602）；湖南省科技计划科研条件创新专项（2013TT1004）；湖南省自然科学基金项目（14J504I）