目的 通过对党参多糖（CPP）的提取纯化工艺优化及其单糖组成和相对分子质量（M）分布进行研究，为进一步分离CPP提供依据。方法 采用硫酸-苯酚法测定多糖的量，采用正交设计确定最佳提取工艺；对提取的粗多糖进行脱蛋白、脱色、透析、冷冻干燥处理，得到精制粗多糖，并采用HPLC和高效凝胶色谱（HPGPC）法，对多糖水解后的单糖组成和相对分子质量分布进行测定。结果 CPP的最佳提取工艺为提取温度85℃，料液比1∶12，提取次数2次，提取时间1.5 h。在上述条件下，CPP的提取率可达到22.57%。水解单糖组成为葡萄糖醛酸、氨基半乳糖、木糖和少量甘露糖，多糖重均相对分子质量（M_w）为21 498。结论 为CPP的分级和活性研究提供了理论基础。

Objective To optimize the extraction process of polysaccharide from Codonopsis pilosula and determine the monosaccharide composition and molecular weight distribution, in order to provide the basis for further separation of C. pilosula polysaccharide. Methods The content of polysaccharide in C. pilosula was determined by phenol sulfuric acid method, the extraction process of polysaccharide was optimized by orthogonal test. C. pilosula polysaccharides were prepared from crude polysaccharides by deproteinization, decoloration, dialysis, and lyophilization, then monosaccharide composition and mean molecular mass of C. pilosula polysaccharides were analyzed by high performance liquid chromatography (HPLC) and high performance gel permeation chromatography (HPGPC). Results The extraction temperature was 85℃, the extraction time was 1.5 h per time, twice, and solid to liquid ratio was 1∶12. Under these conditions, the yield of polysaccharides was 22.57%. The polysaccharides were consisted by glucuronic acid, aminogalactose, xylose, and small quantities of mannose, the average molecular mass was 21 498. Conclusion This study provides a theoretical basis for the classification and activity of polysaccharide from C. pilosula.

山东省中医药科技发展计划项目（2013-227）