目的 考察黄芪甲苷(AS-IV)对人参皂苷CK(GCK)肿瘤细胞摄取和抗肿瘤药效的影响。方法 采用人非小细胞肺癌细胞A549作为肿瘤细胞模型,通过MTT法比较两者配伍与单用GCK的细胞毒性;用荧光法和HPLC法考察了AS-IV与配伍GCK后细胞对GCK的摄取率变化,建立裸鼠荷瘤模型考察AS-IV对GCK的体内增效作用。结果 配伍AS-IV后,GCK对A549细胞的生长抑制作用增强,肿瘤细胞对GCK的摄取增加,且随着AS-IV比例的升高,效果更加显著。荷瘤小鼠实验表明,AS-IV增加了GCK的体内抗肿瘤作用。结论 AS-IV配伍GCK具有增强GCK抗肿瘤药效的作用。

Objective To investigate the effect of astragaloside IV on tumor cellular uptake and antitumor efficacy by ginsenoside compound K (GCK). Methods The human lung adenocarcinoma cell line A549 was prepared as an antitumor model, the cytotoxicity of the mixtures of GCK and astragaloside IV to A549 cells was determined by MTT assay, the cellular uptake of GCK was detected by fluorescence microscopy, and the intracellular GCK was determined by HPLC. The enhancement of astragaloside IV in vivo efficacy by GCK was evaluated by nude mice bearing tumor model. Results The effect of GCK on the inhibition of A549 cell proliferation was enhanced in the presence of astragaloside IV and astragaloside IV could increase the uptake rate of GCK in A549 cells, with the proportion of astragaloside IV increasing, the cytotoxicity was significantly stronger than GCK and the uptake rate was improved. In vivo antitumor assay of mice bearing tumor indicated that astragaloside IV could enhance the antitumor efficacy of GCK. Conclusion Preliminary results indicate that the mixture of GCK and astragaloside IV have the effect of enhancing antitumor efficacy.

国家自然科学基金青年项目(81403119)