目的 研究黄芩醇提物对果蝇寿命的影响,探索黄芩干预果蝇衰老过程中代谢物组的变化规律。方法 用含有不同质量浓度(0.1、1.0、5.0、10.0 g/L)的黄芩醇提物培养基喂养黑腹果蝇,以普通培养基作为对照,观察其对果蝇寿命的影响。分别采集对照组3 d、30 d以及黄芩醇提物(1.0、5.0 g/L)组30 d的果蝇样本进行¹H-NMR检测,采用代谢组学技术及多元统计分析方法研究果蝇组织内源性代谢产物的变化。结果 黄芩醇提物1.0、5.0 g/L能够显著延长雌性果蝇的平均寿命和最高寿命,黄芩醇提物5.0 g/L能显著延长雄性果蝇的平均寿命和最高寿命,并且可以显著升高30 d龄果蝇中异亮氨酸、缬氨酸、醋酸、谷氨酰胺、半胱氨酸、苯丙氨酸、甘氨酸和琥珀酸的量,降低乳酸和葡萄糖的量。结论 黄芩醇提物能够干预机体氨基酸代谢、能量代谢等而起到延缓衰老的作用。代谢组学方法为进一步研究黄芩醇提物延缓衰老的作用机制提供了科学的依据。

Objective To investigate the anti-aging effects and the changes of endogenous metabolites in Drosophila melanogaster by using Scutellariae Radix. Methods Using D. melanogaster (fruit fly) as a model organism and Scutellariae Radix alcohol extract (SAE) as intervention drug, the effect of the drug group on the lifespan of D. melanogaster was observed. The tissue samples of D. melanogaster was collected to analyze the metabolic profile changes among different groups by nuclear magnetic resonance (NMR). Results We demonstrated that SAE could significantly extend the lifespan of fruit fly. Using ¹H-NMR metabonomics and multivariate statistical analysis methods, the changes in the levels of endogenous metabolites in the 30 d D. melanogaster, including decreasing in isoleucine, valine, acetate, glutamine, cysteine, phenylalanine, glycine, and succinate, increasing lactate and glucose, compared with 3 d D. melanogaster. SAE can significantly regulate all of them. Conclusion SAE can regulate amino acid metabolism and energy metabolismto anti-aging. The metabonomic approach is an effective tool for the investigation of the pharmacologic mechanism of SAE and it is helpful to further research.

山西省科技基础条件平台建设项目(2014091022);山西省科技攻关项目(20140313008-14);山西省重点技术创新项目[晋财建-(2013)99号]