目的 探讨蜘蛛香提取物及蜘蛛香总缬草素的抗焦虑作用及相关机制。方法 利用小鼠明暗箱、敞箱、高架十字迷宫和自发性活动实验观察小鼠行为学方面的表现,评价蜘蛛香提取物及总缬草素抗焦虑作用;利用ELISA试剂盒方法测定小鼠血清皮质酮和脑海马组织中神经递质5-羟色胺(5-HT)、去甲肾上腺素(NE)和多巴胺(DA)水平。结果 蜘蛛香提取物及总缬草素各剂量组对小鼠在明暗箱、敞箱、高架十字迷宫和自发性活动实验中的行为学表现均有改善作用,且可降低小鼠血清皮质酮水平和下调脑海马组织中神经递质NE、DA及5-HT的水平。结论 蜘蛛香提取物及总缬草素均有抗焦虑作用,其抗焦虑作用可能是通过作用于丘脑-垂体-肾上腺(HPA)轴系统和调节脑组织神经递质而实现的。

Objective To explore the anti-anxiety effects of the extract and total valeportriate from Valerianae Rhizoma and the relative mechanism. Methods The behaviors of mice were investigated in terms of using in light/dark transition test, open field test, and the elevated plus maze test and spontaneous activity. The the anti-anxiety effect of the extract and total valeportriate from Valerianae Rhizoma have been studied, serum corticosterone and mouse brain hippocampal tissue in the neurotransmitter 5-serotonin (5-HT), norepinephrine (NE) and dopamine (DA) levels were determined by ELISA kit. Results The behavior manifestations of mice in the light/black transition test, open field test, spontaneous activity, and elevated plus maze experiment of mice were improved by the extract and total valeportriate from Valerianae Rhizoma in each dosage group. Test result showed that they could reduce corticosterone level and down-regulate NE, DA, and 5-HT levels. Conclusion The extract and total valeportriate from Valerianae Rhizoma have the anti-anxiety effect and the potential to become the anti-anxiety drug.

全军青年培养项目(13QNP098)