[关键词]
[摘要]
目的 探究桑菊饮的抗炎活性,明确其抗炎活性物质基础;为进一步阐明桑菊饮抗炎作用机制,建立其质量标准提供依据。方法 采用UPLC-Q/TOF技术结合核因子-κB(NF-κB)荧光素酶报告基因检测系统筛选桑菊饮中具有抗炎活性的药效成分,并选取人支气管上皮细胞(BEAS-2B),建立炎症损伤模型,对咖啡酸、绿原酸、甘草酸、马钱子苷和胡萝卜酸等成分的抗炎活性进行验证。结果 筛选出16种具有抑制NF-κB活性的成分,其中胡萝卜酸、22β-乙酰基-甘草酸、甘草皂苷G2抑制NF-κB活性为首次报道。结论 桑菊饮可以通过抑制NF-κB的表达发挥抗炎作用,其抗炎活性主要与16种单体物质相关。
[Key word]
[Abstract]
Objective To investigate the anti-inflammatory activity of Sang Ju Yin (Mulberry Leaf and Chrysanthemum Decoction), and elucidate its bioactive components on nuclear factor-κappa B (NF-κB) inhibition, and to further clarify the anti-inflammatory mechanism of Sang Ju Yin, so as to provide the basis for establishing its quality standard. Methods UPLC-Q/TOF MS coupled with NF-κB activity luciferase reporter assay system was applied to determine the potential anti-inflammatory components in Sang Ju Yin. And the human bronchial epithelial cells (BEAS-2B) were selected to set up the inflammatory injury model, caffeic acid, chlorogenic acid, glycyrrhizic acid, loganin, and malonic acid in Sang Ju Yin were investigated for their anti-inflammation. Results Sixteen components were screened to have the NF-κB inhibitory effects. Among them, malonic acid, 22β-acetoxyl-glycyrrhizic acid, and licorice saponin G2 were first reported to have the NF-κB inhibitory activity. Conclusion Sang Ju Yin contains the potential NF-κB inhibitors. Sixteen potentially active ingredients are found to have the anti-inflammatory effects as NF-κB inhibitors.
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[基金项目]
国家自然科学基金资助项目(81473403)