目的 研究四季青(冬青Ilex chinensis叶)的化学成分。方法 应用多种色谱技术进行分离纯化,根据化合物的波谱数据进行结构鉴定。结果 从四季青70%乙醇提取物中分离得到15个三萜及其皂苷类化合物,分别鉴定为2α,3β-二羟基-23-去甲-4(24)-12(13)-二烯-齐墩果酸(1)、29-羟基常春藤皂苷元(2)、ilexosapogenin A(3)、救必应酸-3,23-缩丙酮(4)、2α,3β-二羟基-23-去甲-4(24)-12-二烯-乌索酸(5)、23-羟基乌索酸(6)、3β,23-二羟基-12,18-二烯-乌索酸(7)、积雪草酸(8)、泰国树脂酸-28-O-β-D-葡萄糖酯苷(9)、28-O-β-D-glucopyranoside spathodic acid(10)、坡模酸-28-O-β-D-葡萄糖酯苷(11)、rotungenoside(12)、3β,23-二羟基-12,18-二烯-乌索酸-28-O-β-D-葡萄糖酯苷(13)、3β,23-二羟基-12,19-二烯-乌索酸-28-O-β-D-葡萄糖酯苷(14)、3β,23-二羟基-12,19(29)-二烯-乌索酸-28-O-β-D-葡萄糖酯苷(15)。结论 化合物1、2为首次从冬青属植物中分离得到,所有化合物均为首次从该植物中分离得到。

Objective To investigate the chemical constituents from the leaves of Ilex chinensis. Methods The constituents were isolated and purified by various chromatographic methods, and the structures were elucidated by spectroscopic analysis. Results Fifteen triterpenoid compounds were isolated from 70% ethanol extract in the leaves of I. chinensis and identified as 2α,3β-dihydroxy-23-norolean-4(24)-12(13)-dien-28-oic acid (1), 29-hydroxyhederagenin (2), ilexosapogenin A (3), rotundic acid isopropylidene (4), 2α,3β-dihydroxy-23-norurs-4(24)-12-dien-28-oic acid (5), 23-hydroxyursolic acid (6), 3β,23-dihydroxyursa-12,18-dien-28-oic acid (7), asiatic acid (8), 28-O-β-D-glucopyranoside siaresinolic acid (9), 28-O-β-D-glucopyranoside spathodic acid (10), 28-O-β-D-glucopyranoside pomolic acid (11), rotungenoside (12), 3β,23-dihydroxyurs-12,18-dien-28-O-β-D-glucopyranosyl ester (13), 3β,23-dihydroxyurs-12,19-dien-28-O-β-D-glucopyranosyl ester (14), and 3β,23-dihydroxyurs-12,19(29)-dien-28-O-β-D-glucopyranosyl ester (15). Conclusion Compounds 1 and 2 are isolated from the plants of Ilex L. for the first time. All compounds are isolated from this plant for the first time.

“十二五”重大专项“中药新药发现与评价技术平台”(2012ZX09301002-002)