目的 研究莛子藨Triosteum pinnatifidum地上部分的化学成分。方法 采用溶剂萃取、薄层色谱以及硅胶、Sephadex LH-20、反相ODS等柱色谱方法进行化合物的分离和纯化,并综合运用¹H-NMR、¹³C-NMR、COSY、HMBC、HSQC、NOESY、MS等波谱手段以及与对照品比对等方法鉴定化合物的结构。结果 从莛子藨地上部分乙醇提取物正丁醇萃取部分中分离得到5个环烯醚萜化合物,分别鉴定为triohima C 10-O-β-D-glucopyranoside(1)、獐芽菜苷(2)、马钱子苷酸(3)、马钱子苷(4)、grandifloroside(5)。结论 化合物1为新化合物,命名为莛子藨苷。

Objective To study the chemical constituents in the aerial parts of Triosteum pinnatifidum. Methods The compounds were separated and purified by solvent extraction, thin-layer chromatography and silica gel, Sephadex LH-20, and ODS column chromatography. Their structures were elucidated by extensive spectroscopic methods including 1D and 2D NMR experiments (¹H-NMR, ¹³C-NMR, COSY, HSQC, HMBC, and NOESY) along with HR-ESI-MS analyses and comparison with reference substances. Results Five iridoids were isolated from the butanol extraction fraction of ethanol extract from the aerial parts of T. pinnatifidum, and identified as triohima C 10-O-β-D-glucopyranoside (1), sweroside (2), loganic acid (3), loganin (4), and grandifloroside (5). Conclusion Compound 1 is a new compound named triopinoside.

教育部新世纪优秀人才支持计划资助项目(NCET-09-0589)