目的 建立透骨草药材来源中狭山野豌豆Vicia amoena超高效液相色谱(UPLC)指纹图谱,并与其余5种来源品种进行比较,为透骨草药材质量控制提供参考依据。方法 用Eclipse Plus C₁₈ RRHD(50 mm×2.1 mm,1.8μm)色谱柱,以乙腈-0.1%甲酸溶液为流动相梯度洗脱,体积流量0.2 mL/min;柱温30℃;检测波长340 nm。测定10个不同产地狭山野豌豆指纹图谱,采用中药色谱指纹图谱相似度评价系统(2004A版)计算相似度,并用对照品和液质联用技术指认色谱峰;同条件下比较鉴别透骨草药材其他5种来源品种。结果 建立了狭山野豌豆指纹图谱,采用超高效液相色谱-电喷雾-四级杆-飞行时间串联质谱仪(UPLC-ESI-Q-TOF-MS)对14个共有峰中的6个峰进行初步归属,确定为绿原酸、杨梅苷、金丝桃苷、异槲皮苷、山柰苷、槲皮苷;透骨草药材其他5种来源品种与狭山野豌豆指纹图谱的共有模式明显不同。结论 该方法重现性好、特征性强,可用于透骨草药材全面质量评价。

Objective To establish the ultra performance liquid chromatography(UPLC) fingerprint of Vicia amoena var.angusta and compare the other five different origin varieties of Viciae Amoenae Herba in order to evaluate the quality of the preparation.Methods Eclipse Plus C₁₈ RRHD column(50 mm×2.1 mm, 1.8μm) were used with acetonitrile(B) and 0.1% formic acid aqueous solution(A) in gradient elution mode at a flow rate of 0.2 mL/min.The detection wavelength was set at 340 nm and the column was 30℃.The fingerprints for V.amoena var.angusta from 10 different areas were set up, the similarity assay for V.amoena var.angusta from ten different areas was carried out to evaluate their quality by Chinese Materia Medica(CMM) Fingerprint Similarity Evaluation System(2004A edition), and the peaks were identified by comparing the reference substance with LC-MS/MS;Whilie the other origins of five kinds of varieties of Viciae Amoenae Herba were compared and identified in the same condition.Results UPLC fingerprints for V.amoena var.angusta were established, six chromatographic peaks among 15 common peaks were identified by UPLC-ESI-Q-TOF-MS and be intended to be chlorogenic acid, myricitrin, hyperoside, isoquercitrin, kaempferit-rin, and quercitrin.UPLC fingerprint mutual pattern of other five kinds of origins varieties of Viciae Amoenae Herba and V.amoena var.angusta had obvious differences.Conclusion This method is simple, rapid, stable, and reproducb1e, and could be used for the full quality evaluation of Viciae Amoenae Herba.

辽宁省中药资源普查-公益性行业科研专项(201407002);辽宁省"十二五"规划"辽宁省中药材标准"编写项目