目的 研究四逆汤及其不同组方配伍对垂体后叶素(Pit)所致大鼠心肌缺血模型的影响。方法 建立大鼠心肌缺血模型,观察四逆汤、附子甘草合煎液、附子干姜合煎液、甘草干姜合煎液、附子单煎液、干姜单煎液、甘草单煎液对大鼠心肌梗死范围、乳酸脱氢酶(LDH)、肌酸激酶(CK)、天冬氨酸转氨酶(AST)、超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、乳酸的影响。结果 四逆汤组、附子甘草组、附子干姜组、附子组能显著减少心肌梗死范围(P<0.01)、减少心肌MDA、乳酸释放(P<0.05、0.01)、降低血清LDH、CK、AST水平(P<0.05、0.01);四逆汤组、附子干姜组能提高心肌SOD活性(P<0.05);四逆汤组能显著提高心肌GSH-Px活性(P<0.01);四逆汤组在心肌LDH水平上低于附子组(P<0.05)。结论 对大鼠缺血心肌的保护程度依次为四逆汤组>附子干姜组>附子甘草组>附子组,从药效的角度揭示了四逆汤配伍的科学性,体现了四逆汤遣方用药之精准。

Objective To study the effects of Sini Decoction before and after compatibilities on acute myocardial ischemia rats. Methods The model of myocardial ischemia in rats was established by ig Pit. The effects of Sini Decoction, Aconiti Lateralis Radix Praeparata (ALRP)-Glycyrrhizae Radix et Rhizoma (GRR) decoction, ALRP-Zingiberis Rhizoma (ZR) decoction, ZR-GRR decoction, single ALRP decoction, single ZR decoction and single GRR decoction on the extension of myocardial infarction, lactic dehydrogenase (LDH), creatine kinase (CK), aspartate aminotransferase (AST), superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), and lactic acid in rats were evaluated. Results Sini Decoction, ALRP-GRR decoction, ALRP-ZR decoction, and single ALRP decoction obviously decreased the extension of myocardial infarction (P<0.01), reduced MDA, lactic acid, LDH, CK, and AST (P<0.01 or P<0.05). Sini Decoction and ALRP-ZR decoction improved SOD activity (P<0.05); Sini Decoction significantly increased GSH-Px activity (P<0.01). The LDH level of Sini Decoction group was lower than that of ingle ALRP decoction (P<0.05). Conclusion Considering various factors, the sequence of the protection of myocardial ischemia in rats is Sini Decoction> ALRP-ZR decoction> ALRP-GRR decoction> single ALRP decoction. It reveals the scientific connotation of the compatibilities of Sini Decoction and accurate action about drug using of Sini Decoction from the perspective of efficacy.

国家自然科学基金项目(81373987)