目的 利用超高效液相色谱-飞行时间质谱联用技术(UPLC/Q-TOF-MS/MS),对中华常春藤Hedera nepalensis茎叶中的化学成分进行分析和鉴定。方法 采用Welch C₁₈色谱柱(100 mm×2.1 mm,1.7μm),以0.1%甲酸水溶液(A)和乙腈(B)为流动相进行梯度洗脱,体积流量0.3 mL/min,柱温40℃,进样量5μL。质谱采用电喷雾(ESI)离子源,在正负离子模式下采集数据,使用质谱分析软件中的目标化合物筛查法,通过保留时间、精确相对分子质量和二级质谱裂解碎片鉴定监测到的化学成分。结果 在优化的液质联用条件下,结合Scifinder数据库、对照品和相关文献鉴定了43个化合物,包括三萜皂苷、黄酮苷类、苯丙素类和核苷类化合物。结论 UPLC/Q-TOF-MS/MS方法能快捷、准确、较全面地鉴定中华常春藤中的化学成分,为中华常春藤化学成分的进一步提取分离和药效物质基础的研究提供科学依据。

Objective Using UPLC/Q-TOF-MS/MS to analyze and identify the chemical constituents from the stems and leaves of Hedera nepalensis. Methods The analysis was performed on an Welch C₁₈ reverse phase column (100 mm×2.1 mm, 1.7 μm). The mobile phase consisted of acetonitrile (B) and 0.1% formic acid (A) was used as gradient elute. The flow rate was 0.3 mL/min gradient elution and column temperature was 40℃, the injection volume was 5 uL, MS conditions were ESI, positive and negative ion mode scanning. Results Under the optimized condition, based on the database of Scifinder, MS/MS of standards and compared with reference results, 43 compounds were identified, including triterpenoid saponins, flavonoid glycosides, phenylpropanoids, and nucleotides. Conclusion By using UPLC/Q-TOF-MS/MS method the main chemical constituents from H. nepalensis can be rapidly and accurately identified.

江西民族传统药现代化科技与产业发展协同创新中心开放基金资助项目(JXXT201402009);江西省青年科学家培养对象项目(20142BCB23022);江西省研究生创新专项资金(YC2014-S290)