目的 研究兴安毛连菜Picris davurica地上部分的化学成分,并进行初步活性评价。方法 采用MCI、Sephadex LH-20和ODS柱色谱分离化合物,运用NMR等波谱法鉴定了化合物结构,通过清除DPPH实验探讨抗氧化活性。结果 从兴安毛连菜60%乙醇提取部分分离得到11个咖啡酸衍生化合物,分别鉴定为咖啡酸(1)、咖啡酰酒石酸(2)、绿原酸(3)、二咖啡酰酒石酸(4)、5-咖啡酰基莽草酸(5)、3,4-二咖啡酰基奎宁酸(6)、3,5-二咖啡酰基奎宁酸(7)、4,5-二咖啡酰奎宁酸(8)、4-咖啡酰基奎宁酸(9)、5-咖啡酰基奎宁酸(10)、3,4-dihydroxy-6-(3,4-dihydroxy-E-styryl)-2-pyron-3-O-β-D-glucopyranoside(11)。结论 所有化合物均为首次从该植物中分离得到,且显示具有一定的抗氧化活性。

Objective To study the chemical constituents from the aerial part of Picris davurica and their anti-oxidative activity. Methods Compounds were isolated by column chromatography of MCI, Sephadex LH-20, and ODS; Their structures and anti-oxidative activity were elucidated by NMR, MS, and DPPH methods. Results On the basis of 1D-NMR, 2D-NMR, and ESI-MS data, eleven compounds were identified as caffeic acid (1), caftaric acid (2), chlorogenic acid (3), chicoric acid (4), 5-O-caffeoylshikimic acid (5), 3,4-di-O-caffeoylquinic acid (6), 3,5-di-O-caffeoylquinic acid (7), 4,5-di-O-caffeoylquinic acid (8) 4-O-caffeoylquinic acid (9), 5-O-caffeoylquinic acid (10), and 3,4-dihydroxy-6-(3,4-dihydroxy-E-styryl)-2-pyron-3-O-β-Dglucopyranoside (11). Conclusion All the compounds reported in this study are isolated from the aerial part of P. hieracioides for the first time and show certain anti-oxidative activity.