目的 观察复方银杏叶颗粒(YXY)对H₂O₂诱导H9c2心肌细胞损伤的影响。方法 采用H₂O₂建立心肌损伤模型,给予YXY 25、50、100、200 μg/mL预处理24 h,MTT法测定细胞生存率,相差显微镜观察细胞形态学改变;试剂盒法检测细胞培养上清中乳酸脱氢酶(LDH)、肌酸激酶-MB(CK-MB)、丙二醛(MDA)、超氧化物歧化酶(SOD)、一氧化氮(NO)水平;共聚焦显微镜检测细胞内活性氧(ROS)、钙离子浓度及线粒体膜电位。结果 H₂O₂ 500 μmol/L作用6 h可显著降低H9c2细胞生存率,诱导氧化应激损伤;YXY预处理可提高细胞生存率,改善损伤细胞形态学改变,减轻LDH、CK外漏,降低细胞上清中MDA、NO水平,增加SOD活性,减少H9c2细胞内ROS生成,降低细胞内钙离子浓度、减轻线粒体膜电位的下降。结论 YXY对H₂O₂诱导H9c2心肌细胞损伤有保护作用。

Objective To study the effects of Compound Granules of Yinxingye (Ginkgo leaf) on H₂O₂-induced cellular injury in H9c2 myocardial cells of rats. Methods H9c2 cells were treated with H₂O₂ to establish a myocardial injury model, and then pretreated by Compound Granules of Yinxingye at the concentration of 25, 50, 100, and 200 μg/mL for 24 h. Cell viability was measured by methyl thiazolyl tetrazolium (MTT). Morphological changes were observed by phase contrast microscope. Lactic dehydrogenase (LDH), creatine kinase-MB (CK-MB), malondialdehyde (MDA), superoxide dismutase (SOD), and nitric oxide (NO) levels were detected by biochemical kits. Intracellular reactive oxygen species and calcium ion concentration and mitochondrial membrane potential were measured by laser confocal microscopy. Results The exposure of H9c2 cells to 500 μmol/L H₂O₂ for 6 h could reduce cell viability significantly and induce oxidative stress injury. The Compound Granules of Yinxingye pretreatment could improve the cell viability, improve the damage cell morphology change, reduce LDH, CK, MDA, and NO levels in the cell supernatant, increase SOD activity. ROS generation and concentration of intracellular calcium could be reduced and the in mitochondrial membrane potential declined. Conclusion The Compound Granules of Yinxingye has the protective effect on H₂O₂-induced cellular injury in H9c2 myocardial cells.

国家国际科技合作项目(S2011ZR0193);科技部第二十次中泰科技合作联委会长期合作项目(20-602J)