目的 研究杜鹃兰Cremastra appendiculata假鳞茎的化学成分。方法 利用正相硅胶柱色谱、葡聚糖凝胶Sephadex LH-20、反相制备液相色谱等方法进行分离纯化,并通过¹H-NMR、¹³C-NMR等波谱技术进行结构鉴定。结果 从杜鹃兰假鳞茎90%乙醇提取物的醋酸乙酯部位中分离得到了11个化合物,分别鉴定为3,5,3'-三羟基联苄(1)、7-羟基-4-甲氧基菲- 2-O-β-D-葡萄糖(2)、lignan glycoside(3)、ibotanolide A(4)、3,3'-二羟基-5-甲氧基-2,4-二(对羟基苄基)-联苄(5)、5,4'-二羟基-二苯乙基-3-O-β-D-葡萄糖苷(6)、3'-β-D-glucopyranosyloxy-4,5'-dihydroxy-3-methoxy-1,2-diphenylethane(7)、2,2',7,7'-四羟基-4,4'-二甲氧基菲-1,1'-二菲(8)、7-羟基-2,4-二甲氧基菲(9)、β-胡萝卜苷(10)、对羟基苯甲醛(11)。结论 化合物3～7为首次从该属植物中分离得到。

Objective To study the chemical constituents from the pseudobulbs of Cremastra appendiculata. Methods The compounds were isolated by repeated column chromatography with silica gel, Sephadex LH-20, and ODS-HPLC. The structures were elucidated by analysis of spectroscopic (¹H-NMR and ¹³C-NMR) data. Results Eleven compounds were isolated from the EtOAc extract in the pseudobulbs of C. appendiculata. Their structures were identified as 3,5,3'-trihydroxybibenzyl (1), 7-hydroxy-4- methoxy-phenanthrene-2-O-β-D-glucoside (2), lignan glycosides (3), ibotanolide A (4), 3,3'-dihydroxy-5-methoxy-2,4-di (p- hydroxybenzyl) bibenzy (5), 5,4'-bihydroxy-bibenzyl-3-O-β-D-glucoside (6), 3'-β-D-glucopyranosyloxy-4,5'-dihydroxy-3-methoxy- 1,2-diphenylethane (7), cirrhopetalanthin (8), 7-hydroxy-2-4-dimethoxy-phenanthrene (9), β-daucosterin (10), and p-hydroxy benzaldehyde (11). Conclusion Compounds 3—7 are isolated from the pseudobulbs of C. appendiculata for the first time.

国家自然科学基金资助项目(31100261);武汉市青年科技晨光计划(2014070404010210)