目的 研究羊开口扦插繁殖的最佳条件。方法 通过4因素3水平正交试验,以生根率、生根数、平均根长和生根指数为指标,研究不同浸泡时间、插穗、激素种类、激素浓度对羊开口扦插生根的影响。在此基础上通过双因素和单因素试验基本摸清羊开口扦插的特性。结果 插穗对羊开口成活率影响最大。当浸泡时间为18 h、嫩枝、激素种类为IBA、处理浓度为0.1 mmol/L时,羊开口扦插的生根效果最好。结论 根据羊开口扦插试验最优组合,可进行羊开口规模化生产,为企业原药材繁育提供技术服务。

Objective To study the best condition for cutting propagation of Melastoma normale. Methods By four factors and three levels orthogonal experiment, using rooting rate, root number, root length, and root mean index as indicators, the effects of different soaking time, cuttings, hormone types, and Melastoma normale rooting hormone concentration on utting propagation of M. normale were investigated. On this basis, through two-factor and single-factor tests to find out the basic characteristics of M. normale cuttings. Results The cuttings had greatest impact on the survival rate of the M. normale. When the soaking time was 18 h, using twigs, hormone types of IBA, and concentration was 0.1 mmol/L, the M. normale rooting cuttings was the best. Conclusion The optimal combination of M. normale cutting experiments can be applied to large-scale production, and providing technical services in crude medicinal materials breeding for enterprises.

桂林市科技攻关项目(20130403-3);桂林“三金片”重要原料羊开口种质资源评价及繁育关键技术研究