目的 建立超顺磁性吸附剂1步高效提取和纯化丹参中药效成分丹参酮的方法。方法 首先采用分散聚合法制备超顺磁性聚羟乙基丙烯酸甲酯(PHEMA)微球,考察其对丹参酮的吸附性能,然后将该微球耦合到丹参酮的提取过程中,考察其1步提取和纯化丹参酮的可行性和分离效率。结果 所制备的超顺磁性PHEMA微球平均粒径为1.2 μm,具有超顺磁性,对丹参酮有着较好的吸附性能;与先提取后纯化的2步法相比,这种1步法不仅能显著增加提取过程的提取效率,对隐丹参酮、丹参酮I和丹参酮II_A的提取率从5 h的0.179、0.093、0.452 mg/g分别提高到0.5 h的0.279、0.176、0.575 mg/g,分离工序少,而且纯化效果好,分离时间也从5.5 h缩短为0.5 h。结论 所提出的1步提取纯化法可行且具有提取效率高、工序少、纯化效果好和分离快速等优点。

Objective A novel one-step extraction and purification method by using superparamagnetic adsorbents was established for separation of tanshinones from Salvia miltiorrhiza. Methods Firstly, superparamagnetic poly hydroxyethyl methacrylate (PHEMA) microspheres were prepared by dispersion polymerization method, and their adsorptive performances of tanshinones were investigated, then these microspheres for purification of tanshinones were coupled into extraction process of tanshinones. The feasibility and separation efficiency of this one-step method were further studied. Results The prepared PHEMA microspheres with the average diameters of 1.2 μm had superparamagnetic property and good adsorption performance for tanshinones. In comparison with two steps method including extraction and purification, one-step method showed significantly higher extraction efficiency of three tanshinones from 0.179, 0.093, and 0.452 mg/g in 5 h to 0.279, 0.176, and 0.575 mg/g in 0.5 h, with less separation process, greatly shorter time consumption from 5.5 h to 0.5 h, and good purification results. Conclusion One-step method proposed is demonstrated feasible and has the merits of high extraction efficiency, less separation process, good purification, and rapid separation speed.

中国博士后科学基金资助项目(2015M572259);湘潭大学博士科研启动项目(14QDZ34)