目的 研究杧果Mangifera indica核仁的化学成分及体外对弱精症大鼠精子质量的影响。方法 采用硅胶柱色谱、SephadexLH-20柱色谱和重结晶等分离纯化手段,分离纯化杧果核仁化学成分,进一步以¹H-NMR、¹³C-NMR和质谱等有机波谱学方法鉴定各单体化合物的结构;采用雷公藤多苷ig大鼠建立弱精症大鼠模型,以此评价化合物体外对弱精症大鼠的精子活力和精子存活率的影响。结果 从杧果核仁中得到9个化合物,分别鉴定为维生素E(1)、没食子酸甲酯(2)、山柰酚(3)、槲皮素(4)、3,4-O-异丙基莽草酸(5)、5-羟基-4-氧代吡喃-2-羧酸(6)、阿魏酸(7)、异槲皮苷(8)、1,2,3,4,6-五-O-没食子酰葡萄糖(9);化合物1、7、9在体外能使弱精症大鼠的精子活力和精子存活率增加。结论 化合物1、3～6、8为首次从杧果核仁中分离得到,化合物1、7、9体外能够改善弱精症大鼠的精子质量。

Objective To study the chemical constituents of mango (Mangifera indica) seed kernel and their effects on sperm quality of asthenospermia rats in vitro. Methods The compounds were isolated and purified by silica gel column chromatography, Sephadex LH-20 column chromatography, and recrystallization. Their structures were elucidated by spectral analyses such as ¹H-NMR, ¹³C-NMR, and MS. The rats were treated with a single ig administration tripterygium glycosides to induce asthenospermia rat model, so that the effects of the compounds could be evaluated on the sperm motility and sperm survival rate of asthenospermia rats in vitro. Results Nine compounds were isolated and purified from mango seed kernel, whose structures were elucidated as vitamin E (1), methyl gallate (2), kaempferol (3), quercetin (4), 3,4-O-isopropylidene shikimic acid (5), 5-hydroxy-4-oxopyran-2-carboxylic acid (6), ferulic acid (7), isoquercitrin (8), and 1,2,3,4,6-penta-O-galloylglucose (9). Compounds 1, 7, and 9 could increase the sperm motility and sperm survival rate of asthenospermia rats in vitro. Conclusion Compounds 1, 3―6 and 8 are obtained from mango seed kernel for the first time; Compounds 1, 7, and 9 could improve the sperm quality of asthenospermia rats in vitro.

广西中药药效研究重点实验室开放基金(13-051-06-K5);四川省科技支撑计划(14ZC0043);广西中医药大学校级课题(K378)