目的 比较黄连、黄柏对正常小鼠机体代谢的影响,探讨二者对小鼠内源性代谢产物的影响规律。方法 采用基于¹H-NMR代谢组学技术结合多元统计分析手段,比较两者对正常小鼠血清和肝脏内源性代谢物干预作用的差异。结果 给药后黄连和黄柏组小鼠机体内源性代谢物的变化既有相似性也有差异性。与对照组比较,给药后小鼠血清中脂类物质、3-羟基丁酸(3-HB)、N-乙酰化糖蛋白(NAG)、甘油、胆碱、α-葡萄糖、β-葡萄糖等代谢物及肝脏中谷氨酸、糖原、腺苷、谷胱甘肽、甜菜碱等代谢物的量均发生变化,但两组的变化幅度不同。内源性代谢物的改变涉及机体的糖代谢、脂肪代谢等途径。结论 黄连、黄柏功效、药性既有相似性,又有差异性。

Objective To compare the effect of Phellodendri Chinensis Cortex (HB) and Coptis Rhizoma (HL) on organism metabolism in mice and explore the regulation of both HB and HL on the endogenous metabolism in mice. Methods ¹H-NMR, based on metabolomic approach coupled with multivariate statistical analysis method, was used to investigate the intervention differences between HB and HL on endogenous metabolites in the serum and liver of normal mice after drug administration. Results The changes of organism endogenous metabolites in both HB and HL treated groups were similar, but the differences also existed. Compared with the control group, the levels of 3-HB, NAG, glycerin, choline, α-glucose,and β-glucose in serum and those of glutamate, glycogen, adenosine, GSSG, betaine, and other metabolites in liver were changed in the two drug treated groups in a different change range. These changes of endogenous metabolites involved in glycometabolism, lipid metabolism, and other pathways. Conclusion This study provides a scientific basis for the comparative analysis of drug function between HB and HL, which has both the similarities and differences.

山西省高等学校创新人才支持计划资助;山西省科技创新重点团队(2013131015)