目的 观察黄精多糖(PSP)对急性心肌梗死(AMI)模型大鼠核因子-κB(NF-κB)介导的炎症反应及心肌组织形态的影响,探讨其对梗死心肌的保护作用及可能机制。方法 采用结扎冠状动脉左前降支的方法制备AMI大鼠模型,假手术组只穿线不结扎,将造模成功的大鼠随机分为模型组,PSP高、中、低剂量(900、450、225 mg/kg)组,麝香保心丸组(12.2 mg/kg),每组10只,每天ig给药1次,连续4周。分别记录手术前、手术后即刻及给药1、3、7、10、14、21、28 d心电图S-T段变化,采用酶联免疫吸附法(ELISA)测定各组大鼠血清肌酸激酶同工酶(CKMB)、乳酸脱氢酶(LDH)、NF-κB、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)的量,并用HE染色观察心肌组织形态病理变化。结果 与模型组比较,PSP能明显抑制AMI模型大鼠心电图S-T段的升高;PSP高、中剂量组大鼠血清CK-MB、LDH及NF-κB、IL-6、TNF-α水平均显著降低(P<0.05、0.01),心肌损伤程度明显改善。结论 PSP能够改善AMI模型大鼠的心肌损伤,减轻炎症反应,修复缺血心肌,其机制可能与调节NF-κB介导的炎症反应有关。

Objective To observe the effect of Polygonatum sibiricum polysaccharides (PSP) on the inflammatory raction mediated by NF-κB and myocardium tissue morphology in acute myocardial infarction (AMI) rats, and to explore the myocardial protective effect and its mechanisms. Methods The AMI model was established by closing the left anterior descending coronary artery of SD rats, Sham group threading without ligation, then the model rats were randomly divided into model group, high-, mid-, low-dose PSP (900, 450, and 225 mg/kg) groups, and positive group (Shexiang protect myocardial pills, 12.2 mg/kg), with 10 rats in each group, ig administered once daily for 28 d. ECG S-T changes were measured before surgery, immediately after surgery, and 1, 3, 7, 10, 14, 21, 28 d after surgery in each group; The levels of inflammatory cytokines IL-6, TNF-α, NF-κB, CKMB, and LDH in serum were measured by ELISA; The myocardial tissue morphology was measured by HE staining. Results Compared with the model group, PSP inhibited S-T segment elevation of ECG in AMI rats; The serum levels of TNF-α, IL-6, NF-κB, CKMB, and LDH in high- and mid-PSP groups decreased significantly (P < 0.05, 0.01), and the degree of myocardial injury was significantly improved. Conclusion PSP could improve the myocardial injury, reduce the inflammation, and repair the ischemic myocardium in AMI rats. The mechanisms may be related to regulating the inflammatory raction mediated by NF-κB.

安徽省研究生"千人培养计划"资助项目