目的 探究4种常用蛋白质测定方法用于中药注射剂中大分子蛋白检测的适用性。方法 比较磺基水杨酸法、考马斯亮蓝法、福林酚(Lowry)法、体积排阻色谱(SEC)法的灵敏度、专属性, 并将4种方法分别用于测定5种市售中药注射剂中的大分子蛋白, 比较测定结果。结果 磺基水杨酸法灵敏度不高, 考马斯亮蓝法和Lowry法的专属性较差, SEC法灵敏度高且专属性好。以SEC法测定5种市售中药注射剂样品, 均未检出大分子蛋白。结论 SEC法可用于中药注射剂中大分子蛋白测定。

Objective To investigate the feasibility of four methods for macromolecule protein determination in Chinese materia medica (CMM) injection. Methods The sensitivity, specificity, and protein test results of sulfosalicylic acid, Coomassie brilliant blue, Lowry, and size exclusion chromatograph (SEC) methods on five kinds of CMM injections were compared. Results Sulfosalicylic acid method had low sensitivity, Coomassie brilliant blue and Lowry methods had poor specificities, and SEC method had high sensitivity and good specificity. No protein was detected in five kinds of CMM injection by SEC. Conclusion SEC is suitable for the determinatoin of macromolecule protein in CMM injection.

国家重大新药创制项目(2013ZX09402203)