目的 对印度木果楝属红树植物Xylocarpus moluccensis种子中的柠檬苦素成分进行研究。方法 利用正、反相硅胶柱以及高效液相等色谱手段进行分离制备, 并利用核磁共振波谱和离子阱电喷雾质谱鉴定化合物结构。结果 从X. moluccensis种子中共分离得到18个柠檬苦素类化合物, 包括14个mexicanolides、2个gedunins、1个andirobine和1个原柠檬苦素;分别鉴定为fissinolide(1)、3-propanoylproceranolide(2)、3β-hydroxyangustidienolide(3)、3β-acetoxy-6-deoxy- swietenine(4)、febrifugin(5)、granatumin I(6)、3-de (2-methylbutanoyl)-3-propanoylcipadesin(7)、granatumin H(8)、tigloylseneganolide A(9)、ruageanin A(10)、swietemahonolide(11)、khayasin T(12)、andhraxylocarpin D(13)、granatumin D(14)、7-oxo-7-deacetoxygenunin(15)、gedunin(16)、methyl angolensate(17)、20,21,22,23-tetrahydro-23-oxoazadirone(18)。结论 化合物2为新化合物, 命名为3-propanoylproceranolide;化合物3为新天然产物。

Objective To investigate limonoids in the seeds of an Indian mangrove, Xylocarpus moluccensis. Methods Compounds were isolated and purified by silica gel, ODS gel column chromatographies, and HPLC. The structures of the obtained limonoids were identified by NMR spectra and ESI-MS. Results Eighteen limonoids, such as fissinolide (1), 3-propanoylproceranolide (2), 3β- hydroxyangustidienolide (3), 3β-acetoxy-6-deoxy-swietenine (4), febrifugin (5), granatumin I (6), 3-de(2-methylbutanoyl)-3- propanoylcipadesin (7), granatumin H (8), tigloylseneganolide A (9), ruageanin A (10), swietemahonolide (11), khayasin T (12), andhraxylocarpin D (13), granatumin D (14), 7-oxo-7-deacetoxygenunin (15), gedunin (16), methyl angolensate (17), and 20,21,22,23- tetrahydro-23-oxoazadirone (18), were isolated and identified. Conclusion 3-propanoylproceranolide (2) is a new compound, and compound 3 is a new natural product.

国家自然科学基金资助项目(81302693, 31170331, 81125022);海洋生物天然产物化合物库(GD2012-D01-001);广东省科技计划项目(2013B051000057)