目的 研究叠鞘石斛Dendrobium aurantiacum var. denneanum药材的化学成分。方法 应用硅胶、大孔吸附树脂AB-8、Sephadex LH-20凝胶柱色谱以及高效液相制备色谱等方法进行分离纯化, 根据质谱和波谱数据鉴定化合物的结构, 同时采用MTT法进行抗肿瘤活性筛选。结果 从叠鞘石斛茎中分离鉴定了9个化合物, 分别为 (-)-(7R,7'R,8R,8'S)-4,4'-二羟基-3,3',5,5'-四甲氧基-7,9'-环氧木脂烷-7',9-内酯(1)、(-)-丁香脂素(2)、(-)-开环异落叶松脂素-4-O-β-D-葡萄糖苷(3)、拖鞋状石斛素(4)、石斛酚(5)、杓唇石斛素(6)、(+)-柚皮素(7)、反式肉桂酸甲酯-2-O-β-D-葡萄糖苷(8)、香草酸(9)。其中, 化合物1对A549、HepG2、A2780肿瘤细胞增殖具有不同程度的抑制作用, IC₅₀分别为5.49、51.2、119.3 μmol/L。结论 化合物1为新化合物, 命名为石斛脂素;且对3种人肿瘤细胞具有一定的细胞毒作用。

Objective To study the chemical constituents from the herb of Dendrobium aurantiacum var. denneanum. Methods The chemical constituents were purified by column chromatography over silica gel, macroporus resin AB-8, Sephadex LH-20, and preparative HPLC, etc. Their structures were identified by spectroscopic data analysis, and their inhibitory activity on tumor cells growth was investigated by MTT method. Results Nine compounds were isolated from D. aurantiacum var. denneanum and identified as (-)-(7R,7'R,8R,8'S)-4,4'-dihydroxy-3,3',5,5'-tetramethoxy-7,9'-epoxylignan-7',9-lactone (1), (-)-syringaresinol (2), (-)-secoisolariciresinol-4-O-β-D-glucopyranoside (3), moscatin (4), gigantol (5), moscatilin (6), (+)-naringenin (7), melilotoside methyl ester (8), and vanillic acid (9). Among them, compound 1 was a new compound and showed the different extent of cytotoxic activities against A549, HepG2, and A2780 tumor cells, with IC₅₀ values of 5.49, 51.2, and 119.3 μmol/L, respectively. Conclusion Compound 1 is a new lignane with the evident cytotoxic activity and named dendroresinol.

"十二五"科技支撑计划(2011BAI13B02-8)