目的 采用化学计量学分析方法, 建立黄芪Astragalus membranaceus药材质量评价体系。方法 收集20批不同产地不同品种黄芪药材, 建立皂苷、黄酮类成分特征图谱。运用主成分分析、聚类分析方法深入研究不同黄芪药材中化学成分量的变化趋势及规律。结果 17个特征成分中, 黄芪皂苷I、黄芪皂苷IV、芒柄花素和2个未知皂苷成分的差异显著, 可作为区分不同产地或不同品种黄芪的关键性指标成分。结论 通过化学计量学分析方法, 建立的黄芪药材中皂苷、黄酮类成分评价体系专属性强、准确性高, 可有效控制和评价黄芪药材质量。

Objective To form the evaluation system of Astragali Radix by chemometrics. Methods To establish the characteristic chromatogram for Astragali Radix with 20 samples from different regions and to analyze the methods data by principal components analysis and cluster analysis. Results There is significant difference in the content of astragaloside I, IV, formononetin, and z6, z7 (unknown components) among the 17 characteristic components. And these components can be the critical index to distinguish the origin. Conclusion It is a sensitivity evaluation system for the quality control of Astragali Radix.

国家重大新药创制(2009ZX09308-001)