目的 对傣药铁刀木Cassia siamea 枝的化学成分进行研究。方法 运用硅胶、凝胶、MCI-gel树脂及RP-HPLC等多种色谱技术进行分离纯化, 并根据理化性质和波谱数据鉴定化合物的结构。结果 从铁刀木枝95%乙醇提取物中分离鉴定了1个蒽醌类化合物, 命名为决明蒽醌 A(1)。生物活性测试中, 其对NB4、SH-SY5Y、PC3、A549和MCF-7的IC₅₀值分别为6.2、7.8、3.5、8.6和5.4 μmol/L。结论 化合物1为新化合物, 并且表现出一定的细胞毒活性。

Objective To study the chemical constituents in the twigs of Cassia siamea. Methods The chemical constituents in the twigs of C. siamea were isolated by silica gel, MCI-gel resin, Sephadex LH-20 column chromatographies and RP-HPLC methods. Their structures were elucidated by spectroscopic methods, including extensive 1D and 2D NMR techniques. Results A new anthraquinone, named as cassanthraquinone A (1) was isolated from 95% ethanol extract of the twigs of C. siamea. Conclusion Compound 1 is a new compound and it displays the cytotoxicity against NB4, SH-SY5Y, PC3, A549, and MCF7 cells with IC₅₀ values of 6.2, 7.8, 3.5, 8.6, and 5.4 μmol/mL, respectively.

国家自然科学基金资助项目(21362044, 21032085);云南省高校科技创新团队资助(IRTSTYN 2014-11)