目的 建立HPLC法同时测定苦丁茶冬青Ilex kudingcha中大叶冬青苷G、大叶冬青苷H、苦丁冬青苷G、大叶冬青苷 C、苦丁冬青苷A、苦丁冬青苷E、苦丁冬青苷D 7个三萜皂苷类成分的量。方法 采用YMC C₁₈色谱柱(YMC-pack ODS-A, 250 mm×4.6 mm, 5 μm), 以乙腈-水为流动相进行梯度洗脱, 体积流量为1.0 mL/min, 检测波长为210 nm, 柱温为30 ℃。结果 7个三萜皂苷化合物均有良好的分离度, 具有良好的线性范围和线性关系(r>0.999 5), 平均加样回收率在99.25%～100.80%, RSD<2%。结论 该方法简单准确, 具有良好的重复性和稳定性, 可为苦丁茶冬青质量控制提供科学依据。

Objective To establish an HPLC method for simultaneously determining latifoloside G, latifoloside H, kudinoside G, latifoloside C, kudinoside A, kudinoside E, and kudinoside D in Ilex kudingcha. Methods The separation was performed on a YMC C₁₈ column (YMC-pack ODS-A, 250 mm × 4.6 mm, 5 μm) with water-acetonitrile as the mobile phase in a gradient elution at a flow rate of 1.0 mL/min. The detection wavelength was 210 nm and the column temperature was 30 ℃. Results Seven triterpenoid saponins all have good separating degree, good linear range, and linearity (r > 0.999 5). The average sample recovery rates were 99.25%—100.8%, RSD < 2%. Conclusion The established method is rapid, accurate, and with has repeatability and stability, which could provides the scientific evidence for the quality control of I. kudingcha.

国家自然科学基金资助项目(81270054, 30901954);广州市珠江科技新星专项(2011J2200047);广东省优秀青年教师培养计划(Yq2013045);广东省自然科学基金资助项目(9451040701003203)