目的 研究怀山药内生真菌厚孢镰刀菌Fusarium chlamydosporum的次生代谢产物。方法 利用硅胶、凝胶等色谱技术进行分离纯化, 通过波谱技术分析鉴定化合物结构。结果 从厚孢镰刀菌的甲醇提取物中分离得到8个化合物, 分别鉴定为过氧麦角甾醇(1)、麦角甾-4,22-二烯-3-酮(2)、邻苯二甲酸二丁酯(3)、邻苯二甲酸二异丁酯(4)、烟酸(5)、琥珀酸(6)、戊二酸(7)和N_b-乙酰基色胺(8)。结论 所有化合物均为厚孢镰刀菌中首次分离得到。

Objective This study focused on the secondary metabolites of endophytic fungus Fusarium chlamydosporum in Dioscorea opposite. Methods Compounds were isolated from the MeOH extract by chromatography technology and their structures were elucidated on the basis of comprehensive spectroscopic analysis. Results Eight compounds were isolated and their structures were identified as: ergosterol-5α,8α-peroside (1), ergosta-4,22-dien-3-one (2), di-n-butyl phthalate (3), di-isobutyl phthalate (4), 3-pyridinecarboxylic acid (5), amber acid (6), pentanedioic acid (7), and N_b-acetyltrytamine (8). Conclusion All these compounds are isolated from F. chlamydosporum for the first time.

河南省教育厅科学技术研究重点项目(14A360006)