目的 研究了傣药香面叶Lindera caudata茎皮的化学成分。方法 运用硅胶、凝胶、MCI-gel树脂及RP-HPLC等多种色谱技术进行分离纯化, 并根据理化性质和波谱数据鉴定化合物的结构, 还用MTT法测定了化合物对NB4、A549、SHSY5Y、PC3和MCF7 5株人癌细胞株的细胞毒活性。结果 从香面叶茎皮中分离鉴定了1个新的8-O-4'-木脂素类化合物, 命名为香面叶木脂素A(1);其对NB4和SHSY5Y细胞的IC₅₀值分别为4.2和5.0 μmol/L。结论 化合物1为新化合物, 其具有显著的细胞毒活性。

Objective To study the chemical constituents in the stem barks of Lindera caudata. Methods The chemical constituents from the stem barks of L. caudata were isolated by column chromatographic methods of silica gel, MCI-Gel resin, Sephadex LH-20, and high performance liquid chromatography. The structures were elucidated by spectroscopic methods, including extensive 1D and 2D NMR techniques. The cytotoxicity of this compound for NB4, A549, SHSY5Y, PC3, and MCF7 cells line was also observed by using the MTT method. Results A 8-O-4'-neolignan, caudatalignan A (1) was isolated from this plants. Conclusion Compound 1 is a new compound and displays the cytotoxicity against NB4 and SHSY5Y cells with IC₅₀ values of 4.2 and 5.0 μmol/L, respectively.

国家自然科学基金资助项目(21362044, 21032085);云南省高校科技创新团队(IRTSTYN 2014-11);云南民族大学-化学与生物技术学院2013年大学生SRT创新性实验项目(2013HXSRTD07);云南民族大学研究生创新项目(2014YJY84)