目的 研究外源激素、水杨酸、茉莉酸甲酯对海巴戟天悬浮细胞生长及蒽醌类化合物合成的影响。方法 建立海巴戟天细胞悬浮培养体系,并向海巴戟天细胞悬浮培养物中添加外源激素、水杨酸和茉莉酸甲酯,测定细胞比生长速率和蒽醌产量。结果 外源激素萘乙酸(NAA)、激动素(KT)对海巴戟天悬浮细胞的生长和蒽醌类化合物的合成有显著影响,采用B5培养基时,较佳激素组合为NAA 2 mg/L+KT 0.1 mg/L。在对数生长初期(培养9 d)时,添加200 μmol/L水杨酸和50 μmol/L茉莉酸甲酯,蒽醌质量浓度分别为588.34 mg/L和595.49 mg/L,比对照分别提高了17.05%和18.47%。结论 适宜激素配比结合一定质量浓度的水杨酸和茉莉酸甲酯外源诱导对蒽醌类化合物的合成有促进作用。

Objective To investigate the effects of exogenous hormones, salicylic acid, and methyl jasmonate on suspension cell growth and anthraquinone production of Morinda citrifolia. Methods After cell suspension lines were established, the cell suspension cultures were treated with exogenous hormones, salicylic acid, and methyl jasmonate, respectively. Then the specific growth rate and anthraquinone production were determined. Results Exogenous hormone NAA and KT had a significant effect on suspension cell growth and anthraquinone production of M. citrifolia. The optimal medium for the cell suspension culture was B5 + 2 mg/L NAA and 0.1 mg/L KT. The anthraquinone content was up to 588.34 and 595.49 mg/L respectively in the presence of 200 μmol/L salicylic acid and 50 μmol/L methyl jasmonate on day 9, and increased by 17.05% and 18.47% respectively more than those in the control group. Conclusion The optimum ratio of exogenous hormones combined with exogenous induction of a certain concentration of salicylic acid and methyl jasmonate could promote the production of anthraquinone.

海南省自然科学基金资助项目(311033)