目的 建立一种知柏地黄丸中盐酸小檗碱(BH)定量测定的方法。方法 以BH为目标分子,甲基丙烯酸(MAA)为功能单体,乙二醇二甲基丙烯酸酯(EGDMA)为交联剂制备出BH分子印迹模板,以此模板为电活性物质,制备出BH玻璃涂膜离子选择性电极,采用工作曲线法测定了知柏地黄丸中BH的量。结果 当BH的浓度在1～100 μmol/L,与电动势(E)呈现良好的Nernst响应,斜率为55.48 mV/pC,回收率为94.5%～105.1%。结论 该方法测定快速、准确、灵敏,结果良好。

Objective To develop a method for the determination of berberine hydrochloride (BH) in Zhibai Dihuang Pills. Methods The BH ion selective electrode was fabricated with a molecularly imprinted polymer (MIP). The process is based on the synthesis of polymers with BH as template, methacrylic acid (MAA) as functional monomer, and ethylene glycol dimethacrylate (EGDMA) as a crosslinker. An ion selective electrode was prepared with this MIP as electric active substance. The content of BH was calculated with working curve. Results The electromotive force (E) exhibited a good Nernst response to BH concentration over a range of 1—100 μmol/L with a slop of 55.48 mV per decade, and recovery rate was 94.5%—105.1%. Conclusion The method is rapid, precise, and accurate, and the result is satisfaction.

黄河科技学院校级项目(KYZR201312)