目的 建立同时测定人参及其制剂中16种人参皂苷的HPLC方法。方法 采用C₁₈（150 mm×4.6 mm，5 μm）色谱柱；流动相为乙腈和水，梯度洗脱，体积流量1.0 mL/min，检测波长203 nm，柱温35℃。结果 16种人参皂苷Rg₁、Re、Rf、Rb₁、Rg₂、Rc、Rb₂、Rb₃、F₁、Rd、F₂、Rg₃、Rh₂及原人参三醇、compound K、原人参二醇均得到良好分离，线性关系良好（r≥0.999 0）。加样回收率均在95%～102%，RSD<2%。结论 该方法快捷简便、稳定可靠，可应用于人参及其制剂的质量控制。

Objective In order to evaluate the quality of Panax ginseng and its preparation, a simple and accurate HPLC method for determining the contents of 16 ginsenosides from P. ginseng was established. Methods The chromatographic separation was achieved on a C₁₈ column (150 mm×4.6 mm, 5 μm) using a mobile phase made up of acetonitrie and water at a flow rate of 1.0 mL/min. The detection wavelength and column temperature were set as 203 nm and 35℃, respectively. Results Sixteen ginsenosides (Rg₁, Re, Rf, Rb₁, Rg₂, Rc, Rb₂, Rb₃, F₁, Rd, F₂, Rg₃, protopanaxatriol, compound K, Rh₂, and protopanaxadiol) were separated at baseline with good linearity (r ≥ 0.999 0). The recovery rates were 95%-102% (RSD < 2%). Conclusion The method is simple, fast, accurate, and could be applied to the quality control of P. ginseng and its preparation.

国家科技支撑计划项目（2011BAI03B010602）；国家公益性行业科研专项（201303111）；国家科技重大专项子课题（2012ZX09304006）； 吉林省基础研究项目（20130102075JC）；吉林省科技厅重点项目（20110228）；吉林省科技条件与平台建设计划（20112101）；吉林省现代农业产业技术体系建设项目（201218）