目的 建立用超高效液相色谱（UPLC）同时测定黄芩中黄芩苷、黄芩素、汉黄芩苷、汉黄芩素、千层纸素A 5种成分的方法。方法 采用UPLC色谱系统，BEH C₁₈色谱柱（50 mm×2.1 mm，1.7 μm）；流动相为乙腈-0.1%甲酸，梯度洗脱，体积流量为0.6 mL/min；进样量为2 μL；检测波长为280 nm。结果 本方法可在15 min内完成1次色谱分析，黄芩苷、黄芩素、汉黄芩苷、汉黄芩素、千层纸素A色谱峰之间有良好的分离度，5种成分的浓度和各自峰面积之间有着良好的线性关系，精密度、重复性及加样回收率的RSD均小于3.0%。结论 本方法快捷、准确，重复性好，能同时测定黄芩中黄芩苷、黄芩素、汉黄芩苷、汉黄芩素、千层纸素A 5种成分的量。

Objective To establish a UPLC method for the simultaneous determination of five constituents (baicalin, wogonoside, baicalein, wogonin, and oroxylin-A) in Scutellaria baicalensis. Methods Five constituents in S. baicalensis were simultaneously determined by UPLC with Waters Acquity UPLC BEH C₁₈ column (50 mm × 2.1 mm, 1.7 μm). With a gradient program of acetonitrile-0.1% formic acid at the flow rate of 0.6 mL/min, and the detection wavelength was set at 280 nm. Results Baicalin, wogonoside, baicalein, wogonin, and oroxylin-A in S. baicalensis were clearly and respectively separated in 15 min. The relationship between the concentration and the peak areas of the five constituents in S. baicalensis was all linear. The RSD of recovery, precision, and reproducibility was all less than 3.0%. Conclusion The method is rapid, simple, reliable, and accurate, and has been successfully used to the quantification of the five constituents in S. baicalensis.

国家自然科学基金资助项目（81072999）