目的 研究热毒宁注射液对A16型柯萨奇病毒（CoxA16）感染Vero细胞及乳鼠模型的抗病毒作用。方法 采用75 TCID₅₀和50 TCID₅₀ CoxA16感染Vero细胞，建立细胞模型；5日龄ICR乳鼠ip感染1×10⁶ TCID₅₀ CoxA16，建立动物模型。观察热毒宁注射液对CoxA16感染Vero细胞病变的影响，体内实验观察热毒宁注射液对病毒感染乳鼠死亡率、生存时间、体质量变化率、临床症状评分的影响。结果 在Vero细胞模型上，热毒宁注射液（生药5.0 mg/mL）显著抑制75 TCID₅₀和50 TCID₅₀ CoxA16引起的细胞病变；在5日龄ICR乳鼠模型上，热毒宁注射液明显缓解CoxA16导致的乳鼠死亡，延长乳鼠生存时间和恢复乳鼠生长抑制；同时热毒宁注射液缓解病毒感染导致的临床症状。结论 热毒宁注射液在体内外均有抗CoxA16作用，为其临床用于治疗手足口病提供依据。

Objective To verify the antiviral effects of Reduning Injection against coxsackievirus A16 (CoxA16) in vivo and in vitro. Methods Vero cells and 5-day-old suckling mice, injected with 75/50 and 10⁶ TCID₅₀ CoxA16, were used as evaluation models. The preventive influences of Reduning Injection against CoxA16 in Vero cells were assessed in the models. The effects of Reduning Injection on the mortality, survival time, change rate of body weight, and clinical symptom scores of suckling mice were observed. Results In Vero cells, cytopathic effects induced by 75 and 50 TCID₅₀ CoxA16 were obviously relieved by crude drug5.0 mg/mL Reduing Injection respectively. Meanwhile, mice death caused by CoxA16 was markedly rescued, survival time was prolonged, and growth inhibition was recovered by Reduing Injection. Meanwhile, the clinical symptom induced by virus was also improved. Conclusion The endogenous and exogenous antivirus effects of Reduning Injection on CoxA16 are proven.

国家科技部重大新药创制项目：现代中药创新集群与数字制药技术平台（2013ZX09402203）