目的 应用动态流变实验评价丹葛眼用即型凝胶及其普通凝胶的流变学特性。方法 以泊洛沙姆为材料制备丹葛眼用温敏即型凝胶，以卡波姆为材料制备其普通凝胶。采用安东帕MCR302流变仪测定上述2种凝胶在不同温度下的流变学参数，并推测即型凝胶的胶凝温度及胶凝时间。结果 丹葛眼用即型凝胶在低温条件下以流体性质为主，黏性模量占主导，为牛顿流体；温度升至室温以上可快速发生相转变，形成凝胶状态，呈假塑性流体，有剪切变稀的性质；该即型凝胶的凝胶温度为（24.4±0.1）℃，胶凝时间为9 s。而丹葛普通凝胶以网状结构存在，在一定温度范围内，凝胶结构稳定，不随温度的变化而改变。结论 建立了丹葛眼用即型凝胶及其普通凝胶的流变学评价体系，运用动态流变学参数可更准确、科学地评价上述2种凝胶的流变学特性，为样品的全面质量控制提供参考依据。

Objective To determine the rheological properties of Dange ophthalmic in-situ gel and its common gel by using dynamic rheological experiments. Methods Dange ophthalmic in-situ gel was prepared by adopting Poloxamer as thermosensitive material, and Dange gel was prepared by carbopol. Anton Paar MCR302 Rheometer was used to determine the rheological parameters of above two kinds of gel at different temperatures which speculated the phase transition time and gelling temperature of in-situ gel. Results Dange ophthalmic in-situ gel was Newtonian liquid at low temperature, with its viscous modulus dominated. It was shear-thinning pseudoplastic fluid under the conditions of phase transition at room temperature, with its elastic modulus dominated. The phase transition temperature (T_g) was (24.4 ± 0.1)℃, and the gelling time was 9 s. Dange gel existed in network structure among a certain temperature range, it was stable and did not change with temperature. Conclusion The test has established the rheological evaluation system of Dange ophthalmic in-situ gel or its common gel, accurately evaluated the rheological properties of the two gels by dynamic rheological parameters, and it can be used as the basis for the quality control of products.

中国中医科学院自主选题（Z02063）；2009年中医药行业科研专项（200907001-5）；北京市共建项目专项资助