目的 通过超高效液相色谱-飞行时间质谱（UPLC/Q-TOF-MS/MS）联用技术，对西南银莲花Anemone davidii根茎的皂苷类化学成分进行鉴定。方法 采用Welch C₁₈色谱柱（100 mm×2.1 mm，1.7 μm），以0.1%甲酸水（A）-乙腈（B）为流动相梯度洗脱进行色谱分离；质谱采用电喷雾（ESI）离子源，在负离子模式下采集数据，运行时间40.25 min，使用质谱分析软件中的目标化合物筛查法，通过保留时间、精确相对分子质量和二级质谱裂解碎片鉴定监测到的化学成分。结果 包括常春藤型皂苷及齐墩果酸型皂苷在内的52个三萜皂苷类成分得到良好的分离和鉴定，47个为该植物中首次发现，其中有9对同分异构体。结论 该方法快速、准确，为西南银莲花的化学成分鉴定提供一种新的策略。

Objective To identify the saponins in the rhizomes of Anemone davidii by ultra performance liquid chromatography coupled with time-of-fight mass spectrometry (UFLC/Q-TOF-MS/MS). Methods The separation was performed on UPLC Welch C₁₈ column (100 mm × 2.1 mm, 1.7 μm), with a mobile phase using 0.1% acetonitrile (A) and water containing 0.1% formic acid (B) for gradient elution. Q-TOF/MS and electrospray ion (ESI) source were applied for the analysis under the negative ion mode, and the running time was 40.25 min. Using target compound screening method, the structures of monitored chemical constituents were identified by retention time, exact relative molecular mass, and cleavage fragments of MS/MS. Results Fifty-two triterpenoids were separated and identified from the methanol extract of A. davidii, 47 triterpenoids were identified for the first time from A. davidii among which nine pairs of structural isomers were included. Conclusion UPLC/Q-TOF-MS/MS method can identify the main chemical constituents from A. davidii rapidly and accurately, which develops a new strategy for identification of the chemical constituents in A. davidii.

贵州省药物代谢研究平台建设项目（黔科合ZY[2011]3013号）；贵州省科技创新人才团队建设项目 [黔科合人才团队（2011）4008]